| Bim and Bmf synergize to induce apoptosis in Neisseria gonorrhoeae infection. | |
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MedLine Citation:
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PMID: 19300516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bcl-2 family proteins including the pro-apoptotic BH3-only proteins are central regulators of apoptotic cell death. Here we show by a focused siRNA miniscreen that the synergistic action of the BH3-only proteins Bim and Bmf is required for apoptosis induced by infection with Neisseria gonorrhoeae (Ngo). While Bim and Bmf were associated with the cytoskeleton of healthy cells, they both were released upon Ngo infection. Loss of Bim and Bmf from the cytoskeleton fraction required the activation of Jun-N-terminal kinase-1 (JNK-1), which in turn depended on Rac-1. Depletion and inhibition of Rac-1, JNK-1, Bim, or Bmf prevented the activation of Bak and Bax and the subsequent activation of caspases. Apoptosis could be reconstituted in Bim-depleted and Bmf-depleted cells by additional silencing of antiapoptotic Mcl-1 and Bcl-X(L), respectively. Our data indicate a synergistic role for both cytoskeletal-associated BH3-only proteins, Bim, and Bmf, in an apoptotic pathway leading to the clearance of Ngo-infected cells. |
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Authors:
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Oliver Kepp; Kathleen Gottschalk; Yuri Churin; Krishnaraj Rajalingam; Volker Brinkmann; Nikolaus Machuy; Guido Kroemer; Thomas Rudel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-20 |
Journal Detail:
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Title: PLoS pathogens Volume: 5 ISSN: 1553-7374 ISO Abbreviation: PLoS Pathog. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-20 Completed Date: 2009-05-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101238921 Medline TA: PLoS Pathog Country: United States |
Other Details:
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Languages: eng Pagination: e1000348 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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metabolism* Apoptosis / physiology* Apoptosis Regulatory Proteins / metabolism* Blotting, Western Cell Adhesion / physiology Cytoskeleton / ultrastructure Flow Cytometry Fluorescent Antibody Technique Gonorrhea / metabolism*, pathology Hela Cells Humans Immunoprecipitation Membrane Proteins / metabolism* Microscopy, Electron, Transmission Mitogen-Activated Protein Kinase 8 / metabolism Neisseria gonorrhoeae / physiology Proto-Oncogene Proteins / metabolism* RNA, Small Interfering Signal Transduction / physiology* rac GTP-Binding Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Apoptosis Regulatory Proteins; 0/BMF protein, human; 0/Bcl-2-like protein 11; 0/Membrane Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Small Interfering; EC 2.7.11.24/Mitogen-Activated Protein Kinase 8; EC 3.6.5.2/rac GTP-Binding Proteins |
| Comments/Corrections | |
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