| Bilirubin diglucuronide formation by rat liver microsomes: demonstration by affinity and thin layer chromatography of bile pigment tetrapyrroles. | |
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MedLine Citation:
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PMID: 6419739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The conjugates formed in vitro by bilirubin UDP-glucuronyl transferase were studied by examining reaction products as intact tetrapyrroles, rather than as dipyrrolic azoderivatives. Bile pigments were extracted from conventional microsomal enzyme reaction mixtures by affinity chromatography over albumin-agarose, eluted with 50% ethanol, and separated by a silica gel thin layer chromatographic system. In the presence of UDPGA, native and activated microsomal preparations all formed both bilirubin mono- and diglucuronides from unconjugated bilirubin, and bilirubin diglucuronide from bilirubin monoglucuronide. No significant non-enzymatic conversion of mono- to diglucuronide occurred without UDPGA, or in the presence of denatured enzyme. Hence, bilirubin diglucuronide is a major product of bilirubin-UDP-glucuronyl transferase. |
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Authors:
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H Okuda; N Tavoloni; C L Kiang; M J Jones; P D Berk |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 117 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1983 Dec |
Date Detail:
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Created Date: 1984-02-20 Completed Date: 1984-02-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 406-12 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bilirubin / analogs & derivatives*, metabolism Chromatography, Affinity Chromatography, Thin Layer Glucuronosyltransferase / metabolism* Male Microsomes, Liver / metabolism* Pyrroles / metabolism* Rats Rats, Inbred Strains Tetrapyrroles |
| Grant Support | |
ID/Acronym/Agency:
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AM-26438/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Pyrroles; 0/Tetrapyrroles; 17459-92-6/bilirubin diglucuronide; 635-65-4/Bilirubin; EC 2.4.1.17/Glucuronosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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