Document Detail


Biliary proteins: assessment of quantitative techniques and comparison in gallstone and nongallstone subjects.
MedLine Citation:
PMID:  3183518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although protein is the third most abundant solid in bile and is important in cholesterol crystal formation, methods for quantitating the concentration of total protein in bile have not been systematically evaluated. To establish a reliable protein assay for bile, we evaluated three protein assays (Lowry's method and the fluorescamine and Coomassie blue methods), and employed amino acid analysis as a reference technique. Large protein-to-protein variations were observed with the fluorescamine and Coomassie blue methods. Although all assays were affected by interfering substances, Lowry's method and the fluorescamine technique (after trichloroacetic acid precipitation and delipidation of bile) and the Coomassie blue method with native bile showed excellent correlations (P less than 0.0001) with those obtained by amino acid analysis. Using these reliable protein assays, we examined gallbladder bile obtained at surgery from subjects with and without gallstones. No differences in the concentrations of total biliary proteins were observed among patients with cholesterol (n = 23) or pigment (n = 7) gallstones and subjects without gallstones (n = 10). Protein values obtained by amino acid analysis also did not differ among groups. As expected, bile from patients with cholesterol gallstones was supersaturated with cholesterol while bile from nongallstone subjects and those with pigment stones was unsaturated. These results indicate that it is not possible to separate patients with and without gallstones on the basis of the total protein concentration of gallbladder bile.
Authors:
K Yamazaki; S P Powers; N F LaRusso
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  29     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-12-16     Completed Date:  1988-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1055-63     Citation Subset:  IM    
Affiliation:
Gastroenterology Research Unit, Mayo Medical School, Rochester, MN 55905.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / analysis*
Cholelithiasis / metabolism*
Humans
Lipids / analysis
Male
Proteins / analysis*
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
DK-24031/DK/NIDDK NIH HHS; DK-34988/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Lipids; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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