| Biliary anionic peptide fraction and apoA-I regulate intestinal cholesterol uptake. | |
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MedLine Citation:
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PMID: 11906174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Evidence is now in favor of protein-facilitated mechanisms for the intestinal cholesterol absorption. Here we report that the unesterified cholesterol uptake by rat jejunal brush border membrane vesicles (BBMVs) is efficient, saturable, and protein-mediated. The human apolipoproteins biliary anionic peptide factor (APF) and A-I (apoA-I) up-regulate micellar cholesterol uptake in a dose-dependent manner, but for all tested concentrations (0.1-20 microM), the lipid-free APF was more efficient than apoA-I. This uptake stimulation was suppressed after addition of Pabs directed to the external lipid-binding domain of the CLA-1/SR-BI and reduced by Pabs directed to the external loop of CD36. Thus, CLA-1/SR-BI and to a lesser extent CD36 are involved in the regulation of intestinal cholesterol uptake. APF, the main protein bound to biliary lipids, is likely one of their physiological effectors. As APF is an unesterified cholesterol carrier, it could facilitate the intestinal absorption of biliary cholesterol. |
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Authors:
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Dominique Jourdheuil-Rahmani; Monique Charbonnier; Nicole Domingo; François Luccioni; Huguette Lafont; Denis Lairon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 292 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-03-21 Completed Date: 2002-05-06 Revised Date: 2004-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 390-5 Citation Subset: IM |
Copyright Information:
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(c)2002 Elsevier Science (USA). |
Affiliation:
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Unit 476-Human Nutrition and Lipids, National Institute for Health and Medical Research (INSERM), Marseille, France. Dominique.Jourdheuil-Rahmani@pharmacie.univ-mrs.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / pharmacology Apolipoprotein A-I / pharmacology* Apoproteins / pharmacology* Calcium-Binding Proteins / pharmacology* Cholesterol / metabolism* Cytoplasmic Vesicles / drug effects, metabolism Dose-Response Relationship, Drug Intestinal Absorption* / drug effects Jejunum / drug effects, metabolism, ultrastructure Kinetics Male Microvilli / drug effects, metabolism Rats Rats, Wistar Receptors, Lipoprotein / antagonists & inhibitors, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Apolipoprotein A-I; 0/Apoproteins; 0/Calcium-Binding Proteins; 0/Receptors, Lipoprotein; 0/anionic polypeptide fraction, human; 0/anionic polypeptide fraction, rat; 57-88-5/Cholesterol |
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