| Biliary sterol secretion is not required for macrophage reverse cholesterol transport. | |
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MedLine Citation:
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PMID: 20620999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent evidence suggests that the intestine may play a direct facilitative role in reverse cholesterol transport (RCT), independent of hepatobiliary secretion. In order to understand the nonbiliary pathway for RCT, we created both genetic and surgical models of biliary cholesterol insufficiency. To genetically inhibit biliary cholesterol secretion, we generated mice in which Niemann-Pick C1-Like 1 (NPC1L1) was overexpressed in the liver. Compared to controls, NPC1L1(Liver-Tg) mice exhibit a >90% decrease in biliary cholesterol secretion, yet mass fecal sterol loss and macrophage RCT are normal. To surgically inhibit biliary emptying into the intestine, we have established an acute biliary diversion model. Strikingly, macrophage RCT persists in mice surgically lacking the ability to secrete bile into the intestine. Collectively, these studies demonstrate that mass fecal sterol loss and macrophage RCT can proceed in the absence of biliary sterol secretion, challenging the obligate role of bile in RCT. |
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Authors:
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Ryan E Temel; Janet K Sawyer; Liqing Yu; Caleb Lord; Chiara Degirolamo; Allison McDaniel; Stephanie Marshall; Nanping Wang; Ramesh Shah; Lawrence L Rudel; J Mark Brown |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell metabolism Volume: 12 ISSN: 1932-7420 ISO Abbreviation: Cell Metab. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-25 Revised Date: 2012-01-25 |
Medline Journal Info:
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Nlm Unique ID: 101233170 Medline TA: Cell Metab Country: United States |
Other Details:
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Languages: eng Pagination: 96-102 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biliary Tract / metabolism* Biological Transport Cholesterol / metabolism* Liver / metabolism Macrophages / immunology, metabolism* Membrane Transport Proteins / genetics, metabolism Mice Mice, Inbred C57BL Mice, Transgenic Models, Animal Sterols / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1K99-HL096166/HL/NHLBI NIH HHS; 5P01HL049373/HL/NHLBI NIH HHS; K99 HL096166-02/HL/NHLBI NIH HHS; P01 HL049373-18/HL/NHLBI NIH HHS; R00 HL096166-04/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Transport Proteins; 0/Npc1l1 protein, mouse; 0/Sterols; 57-88-5/Cholesterol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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