| Bile salt-stimulated lipase of human milk: characteristics of the enzyme in the milk of mothers of premature and full-term infants. | |
| | |
MedLine Citation:
|
PMID: 3430267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human milk contains a lipase (bile salt-stimulated lipase) that is considered to have an important role in infant fat digestion. In this study we compared the characteristics of bile salt-stimulated lipase activity in milk samples from mothers delivering prematurely (26-30 and 31-37 weeks of gestation) and in milk from mothers delivering at term (38-42 weeks of gestation). Preterm milks were collected at day 1-5 and during week 6 of lactation. Term milks were collected during week 6 of lactation. The characteristics of the enzyme (kinetics, enzyme concentration, pH optimum, and pH stability, effects of bile salt structure and concentration, eserine inhibition) were identical regardless of length of pregnancy or duration of lactation. Bile salt-stimulated lipase had a neutral to alkaline pH optimum (pH 7.3-8.6), was stable for 1 h at a wide pH range (pH 3.1-8.6), was active only in the presence of primary bile salts, and was inhibited by eserine. The data indicate that, following parturition at as early as 26 weeks of gestation, the mammary glands synthesizes bile salt-stimulated lipase with identical characteristics as does the mammary gland after a full-term pregnancy. |
| | |
Authors:
|
L M Freed; C M York; P Hamosh; N R Mehta; M Hamosh |
Related Documents
:
|
2702247 - Management of chronic pancreatitis. focus on enzyme replacement therapy. 12929657 - The utility of cyclodextrins in lipase-catalyzed transesterification in organic solvent... 3859917 - Pancreatic duct and microvascular permeability to macromolecules. the relation to acute... 2596427 - Fat emulsion particle size: influence on the clearance rate and the tissue lipolytic ac... 3036087 - The insulin- and glucagon-stimulated 'dense-vesicle' high-affinity cyclic amp phosphodi... 14674717 - Aprt from erythrocytes of hgprt deficient patients: kinetic, regulatory and thermostabi... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of pediatric gastroenterology and nutrition Volume: 6 ISSN: 0277-2116 ISO Abbreviation: J. Pediatr. Gastroenterol. Nutr. Publication Date: 1987 Jul-Aug |
Date Detail:
|
Created Date: 1988-03-15 Completed Date: 1988-03-15 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8211545 Medline TA: J Pediatr Gastroenterol Nutr Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 598-604 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, Georgetown University Medical Center, Washington, D.C. 20007. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bile Acids and Salts
/
physiology Female Humans Hydrogen-Ion Concentration Infant, Newborn Infant, Premature* Kinetics Lipase / analysis* Milk, Human / enzymology* Physostigmine / pharmacology Sterol Esterase* |
| Grant Support | |
ID/Acronym/Agency:
|
HD 2-2816/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bile Acids and Salts; 57-47-6/Physostigmine; EC 3.1.1.-/bile salt-stimulated lipase; EC 3.1.1.13/Sterol Esterase; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of dietary nucleotides on the fatty acid composition of erythrocyte membrane lipids in term ...
Next Document: Vitamin E in human milk from mothers of preterm and term infants.