| Bile salt export pump (BSEP/ABCB11) can transport a nonbile acid substrate, pravastatin. | |
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MedLine Citation:
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PMID: 15901796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pravastatin is a well known 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor. Cumulative studies have shown that pravastatin is taken up into hepatocytes by the organic anion transporting polypeptide family transporters and excreted into the bile as an intact form by multidrug resistance-associated protein 2 (MRP2). It is generally accepted that the bile salt export pump (BSEP/ABCB11) mainly transports bile acids and plays an indispensable role in their biliary excretion. Interestingly, we found that BSEP could accept pravastatin as a substrate. Significant ATP-dependent uptake of pravastatin by human BSEP (hBSEP)- and rat BSEP (rBsep)-expressing membrane vesicles was observed, and the ratio of the uptake activity of pravastatin to that of taurocholic acid (TCA) by hBSEP was 3.3-fold higher than that by rBsep. The K(m) value of pravastatin for hBSEP was 124 muM. A mutual inhibition study between TCA and pravastatin revealed that they competitively interact with hBSEP. Several statins inhibited the hBSEP- and rBsep-mediated uptake of TCA; however, the specific uptake of other statins (cerivastatin, fluvastatin, and pitavastatin) by hBSEP and rBSEP was not detected. The inhibitory effects of hydrophilic statins (pravastatin and rosuvastatin) on the uptake of TCA by BSEP were relatively lower than those of lipophilic statins. These data suggest that BSEP may be partly involved in the biliary excretion of pravastatin in both rats and humans. |
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Authors:
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Masaru Hirano; Kazuya Maeda; Hisamitsu Hayashi; Hiroyuki Kusuhara; Yuichi Sugiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-05-18 |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 314 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-18 Completed Date: 2005-09-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: United States |
Other Details:
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Languages: eng Pagination: 876-82 Citation Subset: IM |
Affiliation:
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Department of Molecular Pharmacokinetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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metabolism* Adenosine Triphosphate / physiology Adenoviridae / metabolism Algorithms Animals Biological Transport, Active Chemistry, Physical Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors / chemistry, metabolism*, pharmacology Kinetics Physicochemical Phenomena Pravastatin / chemistry, metabolism*, pharmacology Rats Taurocholic Acid / metabolism Transport Vesicles / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/ATP-Binding Cassette Transporters; 0/Abcb11 protein, rat; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 56-65-5/Adenosine Triphosphate; 81-24-3/Taurocholic Acid; 81093-37-0/Pravastatin |
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