Document Detail


Bile salt absorption in killifish intestine.
MedLine Citation:
PMID:  3425714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bile salt absorption was examined in vitro using entire small intestines from the killifish Fundulus heteroclitus. Intestines were everted over a glass rod and incubated in solutions containing 10 nM to 20 mM bile salts. After rinsing and correcting for the adherent fluid space, uptake rates and bile salt concentrations in the tissue were determined. The distal intestine contained a Na+-dependent active transport system for bile salt uptake with an apparent Vmax for taurocholate and cholate of 1.4 and 2.3 nmol.min-1.mg dry wt-1 (Km = 117 and 357 microM), respectively. At low concentrations (10 nM to 500 microM), absorption occurred almost exclusively (greater than 84%) in the distal intestine. However, at concentrations of 1 mM and above, bile salt absorption in the middle and proximal regions equaled that in the distal intestine. Thus, although an active transport system makes the distal intestine more efficient in absorbing bile salts, passive absorption appears to account for a significant amount of bile salt uptake at concentrations above the critical micellar concentration. The presence of oleic acid did not significantly affect bile salt uptake.
Authors:
R E Honkanen; J S Patton
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  253     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-01-27     Completed Date:  1988-01-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G730-6     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Georgia, Athens 30601.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / metabolism*
Cholic Acids / metabolism
Cyprinodontiformes / physiology*
Fatty Acids / metabolism
Intestines / anatomy & histology,  metabolism*
Killifishes / physiology*
Sodium / physiology
Taurocholic Acid / metabolism
Grant Support
ID/Acronym/Agency:
AM-01076/AM/NIADDK NIH HHS; AM-27304/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholic Acids; 0/Fatty Acids; 7440-23-5/Sodium; 81-24-3/Taurocholic Acid

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