| Bile proteomic profiles differentiate cholangiocarcinoma from primary sclerosing cholangitis and choledocholithiasis. | |
| | |
MedLine Citation:
|
PMID: 21204070 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Early detection of malignant biliary tract diseases, especially cholangiocarcinoma (CC) in patients with primary sclerosing cholangitis (PSC), is very difficult and often comes too late to give the patient a therapeutic benefit. We hypothesize that bile proteomic analysis distinguishes CC from nonmalignant lesions. We used capillary electrophoresis mass spectrometry (CE-MS) to identify disease-specific peptide patterns in patients with choledocholithiasis (n = 16), PSC (n = 18), and CC (n = 16) in a training set. A model for differentiation of choledocholithiasis from PSC and CC (PSC/CC model) and another model distinguishing CC from PSC (CC model) were subsequently validated in independent cohorts (choledocholithiasis [n = 14], PSC [n = 18] and CC [n = 25]). Peptides were characterized by sequencing. Application of the PSC/CC model in the independent test cohort resulted in correct exclusion of 12/14 bile samples from patients with choledocholithiasis and identification of 40/43 patients with PSC or CC (86% specificity, 93% sensitivity). The corresponding receiver operating characteristic (ROC) analysis revealed an area under the curve (AUC) of 0.93 (95% confidence interval [CI]: 0.82-0.98, P = 0.0001). The CC model succeeded in an accurate detection of 14/18 bile samples from patients with PSC and 21/25 samples with CC (78% specificity, 84% sensitivity) in the independent cohort, resulting in an AUC value of 0.87 (95% CI: 0.73-0.95, P = 0.0001) in ROC analysis. Eight out of 10 samples of patients with CC complicating PSC were identified. Conclusion: Bile proteomic analysis discriminates benign conditions from CC accurately. This method may become a diagnostic tool in future as it offers a new possibility to diagnose malignant bile duct disease and thus enables efficient therapy particularly in patients with PSC. (HEPATOLOGY 2010;). |
| | |
Authors:
|
Tim O Lankisch; Jochen Metzger; Ahmed A Negm; Katja Voβkuhl; Eric Schiffer; Justyna Siwy; Tobias J Weismüller; Andrea S Schneider; Kathrin Thedieck; Ralf Baumeister; Petra Zürbig; Eva M Weissinger; Michael P Manns; Harald Mischak; Jochen Wedemeyer |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-12-7 |
Journal Detail:
|
Title: Hepatology (Baltimore, Md.) Volume: - ISSN: 1527-3350 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2011-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8302946 Medline TA: Hepatology Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Disordered eating and suicidal intent: The role of thin ideal internalisation, shame and family crit...
Next Document: Modeling hepatitis B virus X-induced hepatocellular carcinoma in mice with the sleeping beauty trans...