Document Detail

Bile formation and hepatic plasma membrane composition in guinea-pigs and rats.
MedLine Citation:
PMID:  8574919     Owner:  NLM     Status:  MEDLINE    
We compared bile formation, and biliary and liver plasma membrane composition in guinea-pigs and rats in an attempt to explain the observation that the bile flow rate and the bile acid independent fraction of bile flow (BAIF) in guinea-pigs is about five to seven times higher than in rats. Analysis of electrolytes in bile showed that bicarbonate was significantly [acid] higher in guinea-pigs while Cl-, phosphate and Ca2+ were markedly lower than in rats. High bile independent secretion in guinea-pigs was associated with a significantly lower concentration of total bile acid, phospholipid and cholesterol than in rats. Bile acid distribution studies showed that glycine conjugated chenodeoxycholate and ketolithocholate were the main bile acids in guinea-pigs, while taurine conjugated cholate and muricholate were the predominant bile acids in rats. Total fatty acid analysis of bile indicated that in rats the major fatty acids were palmitic acid (C16:0) and linoleic acid (C18:2, n-6). In guinea-pigs, the contribution of these fatty acids was lower than in rats and compensated with a significantly higher percentage of oleic acid (C18:1, n-9). Concentrations of anionic polypeptide fraction (APF), an acidic calcium binding apoprotein closely associated with biliary phospholipid and cholesterol secretion was also significantly lower in guinea-pigs. Canalicular plasma membrane analysis showed that as compared with rats, specific activities of Na+,K+ ATPase, and cholesterol and phospholipid content were markedly lower in guinea-pigs.(ABSTRACT TRUNCATED AT 250 WORDS)
F Guertin; A Loranger; G Lepage; C C Roy; I M Yousef; N Domingo; F Chanussot; H Lafont; B Tuchweber
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  111     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1996-03-14     Completed Date:  1996-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  523-31     Citation Subset:  IM    
Centre de recherche, Hôpital Ste-Justine, Montréal, Québec, Canada.
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MeSH Terms
Adenosine Triphosphatases / metabolism
Bile / chemistry*,  physiology
Bile Acids and Salts / analysis
Bile Canaliculi / chemistry
Cell Membrane / chemistry*
Guinea Pigs
Liver / metabolism*,  ultrastructure
Membrane Lipids / analysis
Potassium / analysis
Rats, Sprague-Dawley
Sodium / analysis
Reg. No./Substance:
0/Bile Acids and Salts; 0/Membrane Lipids; 7440-09-7/Potassium; 7440-23-5/Sodium; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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