Document Detail


Bile ductular cells undergoing cellular senescence increase in chronic liver diseases along with fibrous progression.
MedLine Citation:
PMID:  20093230     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the pathologic significance of ductular reactions in chronic liver diseases with respect to cellular senescence. The expression of senescence-associated markers (p16(INK4a) and p21(WAF1/Cip1)), cell proliferation, cell cycle markers (cyclin D and cyclin A), and neural cell adhesion molecule (NCAM) was examined immunohistochemically in primary biliary cirrhosis (PBC, n = 37), chronic viral hepatitis (n = 39), nonalcoholic steatohepatitis (n = 25), and control normal livers (n = 12). The expression of p16(INK4a) and p21(WAF1/Cip1) was frequently found in ductular cells in the advanced stage of chronic liver diseases, especially in PBC (P < .05). Double immunostaining disclosed that most senescent cells expressed cyclin D (G(1)-phase marker). NCAM was frequently coexpressed in ductular cells showing senescence-associated markers. Some ductular cells in ductular reactions in chronic liver diseases were at G(1) arrest and undergoing cellular senescence. Such senescent cells may be involved in the progression of fibrosis of these diseases, particularly in PBC.
Authors:
Motoko Sasaki; Hiroko Ikeda; Junpei Yamaguchi; Masami Miyakoshi; Yasunori Sato; Yasuni Nakanuma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  133     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-22     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  212-23     Citation Subset:  AIM; IM    
Affiliation:
Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa 920-8640, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bile Ducts, Intrahepatic / pathology
Biological Markers / analysis*
Cell Aging*
Cell Proliferation
Cyclin D / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Humans
Keratin-7 / metabolism
Liver / metabolism
Liver Cirrhosis, Biliary / metabolism,  pathology*
Liver Diseases / metabolism,  pathology*
Neoplasm Proteins / metabolism
Neural Cell Adhesion Molecules / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/CDKN1A protein, human; 0/Cyclin D; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Keratin-7; 0/Neoplasm Proteins; 0/Neural Cell Adhesion Molecules; 0/P16 protein, human

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