Document Detail


Bile acid inhibition of vitamin B12 binding by intrinsic factor in vitro.
MedLine Citation:
PMID:  7239318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of conjugated and unconjugated bile acids on the binding of vitamin B12 to intrinsic factor was investigated. The dihydroxy bile acids (deoxycholic, glycodeoxycholic, taurodeoxycholic, glycochenodeoxycholic, and taurochenodeoxycholic) inhibit the binding of intrinsic factor to vitamin B12 at physiological concentrations. On the other hand, the trihydroxy bile acids (cholic, glycocholic, and taurocholic) are not effective in this respect. The inhibition is dependent both on concentration and time, and its pattern is similar to that previously reported for duodenal juice. On column chromatography, there is a close correlation between the degree in intrinsic factor inhibition and the total acid concentration in the duodenal juice. The binding of vitamin B12 by R protein in saliva is not affected by bile acids. The results show that bile acids at concentrations found in duodenal juice inhibit intrinsic factor vitamin B12 binding. It is suggested that this observation may have physiological significance for vitamin B12 absorption.
Authors:
N H Teo; J M Scott; B Reed; G Neale; D G Weir
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gut     Volume:  22     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  270-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / pharmacology*
Clusterin
Duodenum / metabolism
Gastric Juice / metabolism
Glycoproteins / metabolism
Humans
Intestinal Secretions / metabolism
Intrinsic Factor / metabolism*
Molecular Chaperones*
Protein Binding / drug effects
Saliva / metabolism
Salivary Proteins and Peptides / metabolism
Vitamin B 12 / metabolism*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/CLU protein, human; 0/Clusterin; 0/Glycoproteins; 0/Molecular Chaperones; 0/Salivary Proteins and Peptides; 68-19-9/Vitamin B 12; 9008-12-2/Intrinsic Factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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