| Big ANF: large-molecular-weight ANF in plasma. I. Gel filtration and affinity chromatography studies. | |
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MedLine Citation:
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PMID: 1833983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A large molecular form of immunoreactive atrial natriuretic factor (irANF) was demonstrated in plasma of rabbit and rat on the basis of gel filtration experiments. This big ANF was not retained by octadecylsilane cartridges and cross-reacted with four anti-ANF antisera of different specificities. Gel filtration in acid, but not in 8 M urea, resulted in material with elution characteristics of irANF. Affinity chromatography and gel electrophoresis of big ANF suggested that the material was similar to albumin. However, high concentrations of big ANF were found in analbuminemic rats, with characteristics similar to those seen in rabbit and normal rats (affinity and gel chromatography and gel electrophoresis). We thus conclude that big ANF represents a bound form of ANF in circulation and that the carrier is similar to but not identical with albumin. |
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Authors:
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N Wilson; V Yakoleff; R Keeler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 261 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-11-18 Completed Date: 1991-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E516-24 Citation Subset: IM |
Affiliation:
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Department of Physiology, Faculty of Medicine, University of British Columbia, Vancouver, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid Acetic Acids Animals Atrial Natriuretic Factor / blood, chemistry* Buffers Chromatography, Affinity Chromatography, Gel Electrophoresis, Polyacrylamide Gel Humans Molecular Weight Phosphates Rabbits Radioimmunoassay Rats Rats, Inbred Strains Rats, Mutant Strains Serum Albumin / analysis Urea |
| Chemical | |
Reg. No./Substance:
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0/Acetic Acids; 0/Buffers; 0/Phosphates; 0/Serum Albumin; 57-13-6/Urea; 64-19-7/Acetic Acid; 85637-73-6/Atrial Natriuretic Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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