Document Detail

Bifidobacterium breve enhances transforming growth factor beta1 signaling by regulating Smad7 expression in preterm infants.
MedLine Citation:
PMID:  16819382     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Transforming growth factor (TGF) beta1 displays a broad spectrum of activities in mucosal regulation, including induction of oral tolerance, potent anti-inflammatory effects, mucosal IgA expression and effects on epithelial cell proliferation and differentiation. The present study examined the effect of probiotics on the immunologic system of preterm infants in relation to TGF-beta signaling. METHODS: Subjects comprised 19 preterm infants divided into 2 groups: receiving Bifidobacterium breve supplementation (B. breve group) and without supplementation (controls). Blood samples were collected from both groups on days 0, 14 and 28 after birth. Serum cytokine levels were measured using enzyme-linked immunosorbent assay, and expression levels of the TGF-beta signaling molecule, Smad, were examined using semiquantitative reverse transcriptase-polymerase chain reaction. RESULTS: Serum TGF-beta1 level was elevated on day 14 and remained elevated on day 28 in the B. breve group. Level of messenger RNA expression was enhanced for Smad3 and reduced for Smad7 (antagonistic Smad) after B. breve administration relative to levels in controls on day 28. CONCLUSIONS: These results demonstrated that the administration of B. breve to preterm infants can up-regulate TGF-beta1 signaling and may possibly be beneficial in attenuating inflammatory and allergic reactions in these infants.
Tohru Fujii; Yoshikazu Ohtsuka; Tsubasa Lee; Takahiro Kudo; Hiromichi Shoji; Hiroaki Sato; Satoru Nagata; Toshiaki Shimizu; Yuichiro Yamashiro
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  43     ISSN:  0277-2116     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-04     Completed Date:  2007-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-8     Citation Subset:  IM    
Department of Pediatrics and Adolescence Medicine, Juntendo University School of Medicine, Tokyo, Japan.
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MeSH Terms
Biological Markers / blood
Cytokines / blood
Enzyme-Linked Immunosorbent Assay
Immune System / metabolism
Infant, Newborn
Infant, Premature*
Intestinal Mucosa / metabolism
Probiotics / administration & dosage*
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Smad3 Protein / biosynthesis
Smad7 Protein / biosynthesis*
Time Factors
Transforming Growth Factor beta1 / biosynthesis*
Reg. No./Substance:
0/Biological Markers; 0/Cytokines; 0/RNA, Messenger; 0/Smad3 Protein; 0/Smad7 Protein; 0/Transforming Growth Factor beta1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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