Document Detail

Bidirectional ion transport in thyroid: secretion of anions by monolayer cultures that absorb sodium.
MedLine Citation:
PMID:  1733249     Owner:  NLM     Status:  MEDLINE    
Cultured porcine thyroid cell monolayers transport Na+ in an apical-to-basal direction, resulting in the development of a basal-positive transepithelial potential difference (TEP) and the formation of domes (fluid-filled elevations of the cell layer above the culture dish substrate). Stimulation by prostaglandin E2 (PGE2) increases the magnitude of the TEP, the short-circuit current (Isc) measured in Transwell Ussing chambers, and the height of domes in cultures grown on impermeable substrates. A phenamil-resistant, PGE2-stimulated component of the Isc in Transwells and of the TEP in monolayers in conventional culture dishes was inhibitable by bumetanide, a diuretic drug that blocks NaKCl2 symporters, mediating active transport of Cl-. The rate of decrease in height of domes in cultures after addition of phenamil, presumably indicative of transport of fluid in a basal-to-apical direction, was also reduced by bumetanide. Studies with Transwells in Cl(-)-free, HCO(3-)-free or Cl(-)- and HCO(3-)-free media indicated that thyroid cells transported HCO3- as well as Cl- in a basal-to-apical direction. It was concluded that the thyroid epithelium is both sodium absorbing and anion secreting.
J Armstrong; T Matainaho; E J Cragoe; G J Huxham; J R Bourke; S W Manley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  262     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-02-21     Completed Date:  1992-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E40-5     Citation Subset:  IM    
Department of Physiology and Pharmacology, University of Queensland, St Lucia, Australia.
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MeSH Terms
Amiloride / analogs & derivatives,  pharmacology
Anions / metabolism*
Bicarbonates / pharmacology
Biological Transport
Bumetanide / pharmacology
Cells, Cultured
Chlorides / pharmacology
Cytological Techniques
Dinoprostone / pharmacology
Drug Resistance
Epithelial Cells
Epithelium / metabolism,  physiology
Sodium / pharmacokinetics*
Thyroid Gland / cytology,  metabolism*,  physiology
Reg. No./Substance:
0/Anions; 0/Bicarbonates; 0/Chlorides; 0/Ions; 2038-35-9/phenylamil; 2609-46-3/Amiloride; 28395-03-1/Bumetanide; 363-24-6/Dinoprostone; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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