Document Detail


Biciliated ependymal cell proliferation contributes to spinal cord growth.
MedLine Citation:
PMID:  22434575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two neurogenic regions have been described in the adult brain, the lateral ventricle subventricular zone and the dentate gyrus subgranular zone. It has been suggested that neural stem cells also line the central canal of the adult spinal cord. Using transmission and scanning electron microscopy and immunostaining, we describe here the organization and cell types of the central canal epithelium in adult mice. The identity of dividing cells was determined by 3D ultrastructural reconstructions of [(3) H]thymidine-labeled cells and confocal analysis of bromodeoxyuridine labeling. The most common cell type lining the central canal had two long motile (9+2) cilia and was vimentin+, CD24+, FoxJ1+, Sox2+, and CD133+, but nestin- and glial fibrillary acidic protein (GFAP)-. These biciliated ependymal cells of the central canal (Ecc) resembled E2 cells of the lateral ventricles, but their basal bodies were different from those of E2 or E1 cells. Interestingly, we frequently found Ecc cells with two nuclei and four cilia, suggesting they are formed by incomplete cytokinesis or cell fusion. GFAP+ astrocytes with a single cilium and an orthogonally oriented centriole were also observed. The majority of dividing cells corresponded to biciliated Ecc cells. Central canal proliferation was most common during the active period of spinal cord growth. Pairs of labeled Ecc cells were observed within the central canal in adult mice 2.5 weeks post labeling. Our work suggests that the vast majority of postnatal dividing cells in the central canal are Ecc cells and their proliferation is associated with the growth of the spinal cord.
Authors:
Clara Alfaro-Cervello; Mario Soriano-Navarro; Zaman Mirzadeh; Arturo Alvarez-Buylla; Jose Manuel Garcia-Verdugo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  520     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-08-17     Completed Date:  2013-06-10     Revised Date:  2013-09-24    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3528-52     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, 46980, Valencia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Cilia / physiology,  ultrastructure
Ependyma / cytology*,  physiology
Mice
Mice, Inbred Strains
Neural Stem Cells / physiology,  ultrastructure*
Spinal Cord / cytology*,  growth & development*
Grant Support
ID/Acronym/Agency:
HD32116/HD/NICHD NIH HHS; NS28478/NS/NINDS NIH HHS; R01 NS028478-18/NS/NINDS NIH HHS; R01 NS028478-19/NS/NINDS NIH HHS; R01 NS028478-20/NS/NINDS NIH HHS; R37 HD032116-16/HD/NICHD NIH HHS; R37 HD032116-17/HD/NICHD NIH HHS; R37 HD032116-18/HD/NICHD NIH HHS
Comments/Corrections

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