Document Detail


Biaxial strain properties of elastase-digested porcine aortic valves.
MedLine Citation:
PMID:  10888104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS OF THE STUDY: Previous studies have suggested that elastin in porcine aortic valve cusps is responsible for restoring collagen fibers to their original configuration between loading-unloading cycles. METHODS: Biaxial loading tests were performed on intact aortic valves before and after elastase treatment to further investigate the role of elastin. RESULTS: Degradation of elastin caused an increase in the radial dimensions of the cusps (mean increase in gauge length, 29%), which corresponded to a significant decrease in radial extensibility (mean decrease, 61%) and a threefold increase in radial stiffness. Changes in circumferential extensibility and stiffness were smaller and, for most cusps, were not statistically significant. Control experiments, in which the valves were treated with buffer only, resulted in the opposite changes in radial dimensions and extensibility (7% decrease in gauge length and doubling of extensibility). CONCLUSION: Changes in the mechanical properties of the aortic valve cusps following incubation in elastase were due to elastin damage, and not incidental to soaking in buffer. As many explanted bioprosthetic valves have mechanical characteristics similar to those of the elastase-treated valves, elastin damage may be a factor in the progressive degeneration and ultimate failure of bioprosthetic heart valves.
Authors:
M M Adamczyk; T C Lee; I Vesely
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of heart valve disease     Volume:  9     ISSN:  0966-8519     ISO Abbreviation:  J. Heart Valve Dis.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-10-27     Completed Date:  2000-10-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9312096     Medline TA:  J Heart Valve Dis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  445-53     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, The Lerner Research Institute, The Cleveland Clinic Foundation, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aortic Valve / physiology,  transplantation*
Bioprosthesis*
Elastin / metabolism*,  physiology
Heart Valve Prosthesis*
Pancreatic Elastase* / pharmacology
Prosthesis Failure
Stress, Mechanical
Swine
Grant Support
ID/Acronym/Agency:
R01 HL57236/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-58-3/Elastin; EC 3.4.21.36/Pancreatic Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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