Document Detail


Biasing amacrine subtypes in the Atoh7 lineage through expression of Barhl2.
MedLine Citation:
PMID:  23035102     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Within the developing vertebrate retina, particular subtypes of amacrine cells (ACs) tend to arise from progenitors expressing the basic helix-loop-helix (bHLH) transcription factor, Atoh7, which is necessary for the early generation of retinal ganglion cells (RGCs). All ACs require the postmitotic expression of the bHLH pancreas transcription factor Ptf1a; however, Ptf1a alone is not sufficient to give subtype identities. Here we use functional and in vivo time-lapse studies in the zebrafish retina to investigate on the developmental programs leading to ACs specification within the subsequent divisions of Atoh7-positive progenitors. We find evidences that the homeobox transcription factor Barhl2 is an AC subtype identity-biasing factor that turns on within Atoh7-positive descendants. In vivo lineage tracing reveals that particular modes of cell division tend to generate Barhl2-positive precursors from sisters of RGCs. Additionally, Atoh7 indirectly impacts these division modes to regulate the right number of barhl2-expressing cells. We finally find that Atoh7 itself influences the subtypes of Barhl2-dependent ACs. Together, the results from our study uncover lineage-related and molecular logic of subtype specification in the vertebrate retina, by showing that specific AC subtypes arise via a particular mode of cell division and a transcriptional network cascade involving the sequential expression of first atoh7 followed by ptf1a and then barhl2.
Authors:
Patricia R Jusuf; Shahad Albadri; Alessio Paolini; Peter D Currie; Francesco Argenton; Shin-ichi Higashijima; William A Harris; Lucia Poggi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  32     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-04     Completed Date:  2013-01-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13929-44     Citation Subset:  IM    
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Victoria 3800, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amacrine Cells / classification,  cytology*,  metabolism
Animals
Animals, Genetically Modified
Cell Division
Cell Lineage
DNA-Binding Proteins / genetics,  physiology*
Female
Gene Expression Regulation, Developmental / drug effects
Helix-Loop-Helix Motifs / physiology
Male
Morpholinos / pharmacology
Retina / embryology
Time-Lapse Imaging
Transcription Factors / biosynthesis,  genetics,  physiology*
Transcription, Genetic / drug effects
Zebrafish
Zebrafish Proteins / biosynthesis,  genetics,  physiology*
Grant Support
ID/Acronym/Agency:
083203//Wellcome Trust; RG49253//Wellcome Trust
Chemical
Reg. No./Substance:
0/Barhl2 protein, zebrafish; 0/DNA-Binding Proteins; 0/Morpholinos; 0/Transcription Factors; 0/Zebrafish Proteins; 0/ath5 protein, zebrafish; 0/transcription factor PTF1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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