| BiP-dependent export of cholera toxin from endoplasmic reticulum-derived microsomes. | |
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MedLine Citation:
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PMID: 14623108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cholera toxin (CT) is transported from the cell surface to the endoplasmic reticulum (ER) from where it is translocated to the cytosol in a process depending on ATP and luminal ER proteins. To test whether the molecular chaperone BiP (heavy chain binding protein), which is an ER-luminal ATPase, was one of the required proteins the export of CT was analyzed using ER-derived CT-loaded microsomes. The resubstitution of extracted export-incompetent microsomes with purified BiP was sufficient to restore the export of CT. As BiP protected CT from aggregation it is proposed that BiP maintains CT in a soluble, export-competent state. |
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Authors:
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Alexandra Winkeler; Daniela Gödderz; Volker Herzog; Anton Schmitz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 554 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-11-18 Completed Date: 2003-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 439-42 Citation Subset: IM |
Affiliation:
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Institut für Zellbiologie, Rheinische Friedrich-Wilhelms Universität Bonn, Ulrich-Haberland-Str. 61a, 53121 Bonn, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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genetics,
metabolism,
pharmacology Animals Biological Transport CHO Cells Carrier Proteins / genetics, metabolism*, pharmacology Cholera Toxin / chemistry, genetics, metabolism* Cricetinae Endoplasmic Reticulum / metabolism* Heat-Shock Proteins* Immunoblotting Microsomes / metabolism*, ultrastructure Molecular Chaperones / genetics, metabolism*, pharmacology Protein Folding Protein Processing, Post-Translational Recombinant Proteins / genetics, metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Heat-Shock Proteins; 0/Molecular Chaperones; 0/Recombinant Proteins; 0/molecular chaperone GRP78; 9012-63-9/Cholera Toxin; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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