| Betagamma subunits mediate the NPY enhancement of ATP-stimulated inositol phosphate formation. | |
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MedLine Citation:
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PMID: 15063008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neuropeptide Y (NPY) enhances ATP-stimulated inositol phosphate (InsP) formation in bovine chromaffin cells through an unknown mechanism. Chromaffin cells were transduced with the carboxyl terminus of beta-adrenergic receptor kinase 1 (betaARK1CT), a Gbetagamma subunits scavenger, using a recombinant adenovirus system. The adenovirus also expresses a green fluorescent protein (GFP) which serves as an index of transduction. Flow cytometry showed that up to 80% of chromaffin cells were transduced by the virus. There was a direct correlation between the betaARK1CT inhibition of the NPY enhancement of ATP-stimulated InsP formation and the percent of cells expressing GFP ( r2=0.9993 ). These results demonstrate that Gbetagamma subunits are required for the NPY enhancement of ATP-stimulated InsP formation in bovine chromaffin cells. |
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Authors:
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Xinying Li; Tsuneya Ikezu; Terry D Hexum |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Peptides Volume: 25 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-04-05 Completed Date: 2004-11-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: United States |
Other Details:
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Languages: eng Pagination: 267-74 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, 986260 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198-6260, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism* Adenoviridae / genetics Animals Cattle Cells, Cultured Chromaffin Cells / metabolism* Cyclic AMP-Dependent Protein Kinases / metabolism* Green Fluorescent Proteins Inositol Phosphates / metabolism* Luminescent Proteins / metabolism Neuropeptide Y / metabolism* beta-Adrenergic Receptor Kinases |
| Grant Support | |
ID/Acronym/Agency:
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HL65135/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Luminescent Proteins; 0/Neuropeptide Y; 147336-22-9/Green Fluorescent Proteins; 56-65-5/Adenosine Triphosphate; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.15/beta-Adrenergic Receptor Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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