Document Detail

BetaPIX and GIT1 regulate HGF-induced lamellipodia formation and WAVE2 transport.
MedLine Citation:
PMID:  19303398     Owner:  NLM     Status:  MEDLINE    
Formation of lamellipodia is the first step during cell migration, and involves actin reassembly at the leading edge of migrating cells through the membrane transport of WAVE2. However, the factors that regulate WAVE2 transport to the cell periphery for initiating lamellipodia formation have not been elucidated. We report here that in human breast cancer MDA-MB-231 cells, the hepatocyte growth factor (HGF) induced the association between the constitutive complex of betaPIX and GIT1 with WAVE2, which was concomitant with the induction of lamellipodia formation and WAVE2 transport. Although depletion of betaPIX by RNA interference abrogated the HGF-induced WAVE2 transport and lamellipodia formation, GIT1 depletion caused HGF-independent WAVE2 transport and lamellipodia formation. Collectively, we suggest that betaPIX releases cells from the GIT1-mediated suppression of HGF-independent responses and recruits GIT1 to WAVE2, thereby facilitating HGF-induced WAVE2 transport and lamellipodia formation.
Shigeru Morimura; Katsuo Suzuki; Kazuhide Takahashi
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Publication Detail:
Type:  Journal Article     Date:  2009-03-19
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  382     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-15     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  614-9     Citation Subset:  IM    
Molecular Cell Biology Division, Kanagawa Cancer Center Research Institute, 1-1-2 Nakao, Asahi-ku, Yokohama 241-0815, Japan.
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MeSH Terms
Adaptor Proteins, Signal Transducing / metabolism*
Cell Cycle Proteins / metabolism*
Cell Line, Tumor
Cell Movement*
Guanine Nucleotide Exchange Factors / metabolism*
Hepatocyte Growth Factor / metabolism*,  pharmacology
Protein Transport
Pseudopodia / metabolism,  physiology*
Wiskott-Aldrich Syndrome Protein Family / metabolism*
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Cell Cycle Proteins; 0/GIT1 protein, human; 0/Guanine Nucleotide Exchange Factors; 0/HGF protein, human; 0/WASF2 protein, human; 0/Wiskott-Aldrich Syndrome Protein Family; 0/rho guanine nucleotide exchange factors; 67256-21-7/Hepatocyte Growth Factor

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