Document Detail


BetaIg-h3 is involved in the HAb18G/CD147-mediated metastasis process in human hepatoma cells.
MedLine Citation:
PMID:  17327467     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HAb18G/CD147, a new hepatoma-associated antigen cloned and screened from human hepatocellular carcinoma cDNA library, is closely correlated with metastasis process in human hepatoma cells. In the present study we aimed to identify the pivotal molecules of the HAb18G/CD147 signal transduction pathway. The investigation showed that betaig-h3, a secretory extracellular matrix (ECM) protein, was upregulated in HAb18G/CD147-expressing human hepatoma T7721 cells and was downregulated by depressing HAb18G/CD147 expression. The expression of betaig-h3, upregulated in human hepatoma cells, was positively relative to the expression of HAb18G/CD147 in different human hepatoma cell lines. By overexpressing betaig-h3 in human SMMC-7721 hepatoma cells, we discovered that betaig-h3 promoted cell adhesion, invasion, and matrix metalloproteinase (MMP) secretion potential. HAb18G/CD147-induced invasion and metastasis potential of human hepatoma cells can be attenuated by antibodies specific for betaig-h3, and no significant differences on inhibitory effects were observed among T7721 cells incubated with antibodies for betaig-h3 or HAb18G/CD147 or both types together. Taken together, our study suggests that betaig-h3, regulated by the expression of HAb18G/CD147, is involved in the HAb18G/CD147 signal transduction pathway and mediates the HAb18G/CD147-induced invasion and metastasis process of human hepatoma cells.
Authors:
Juan Tang; Hong-wei Zhou; Jian-li Jiang; Xiang-min Yang; Yu Li; Hong-xin Zhang; Zhi-nan Chen; Wei-ping Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  232     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-28     Completed Date:  2007-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  344-52     Citation Subset:  IM    
Affiliation:
Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, 17 Changlexi Street, Xi'an 710032, China.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / pharmacology
Antigens, CD147 / genetics,  immunology,  metabolism*
Carcinoma, Hepatocellular / genetics,  metabolism,  pathology
Cell Adhesion / drug effects,  genetics
Cell Line
Cell Line, Tumor
Down-Regulation / genetics
Extracellular Matrix Proteins / genetics,  immunology,  metabolism*
Gene Expression Profiling
Gene Expression Regulation, Neoplastic / drug effects
Humans
Liver Neoplasms / genetics,  metabolism,  pathology
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Neoplasm Invasiveness
Neoplasm Metastasis
Oligonucleotide Array Sequence Analysis
RNA, Small Interfering / genetics
Transfection
Transforming Growth Factor beta / genetics,  immunology,  metabolism*
Up-Regulation / genetics
Chemical
Reg. No./Substance:
0/Antibodies; 0/BSG protein, human; 0/Extracellular Matrix Proteins; 0/RNA, Small Interfering; 0/Transforming Growth Factor beta; 136894-56-9/Antigens, CD147; 148710-76-3/betaIG-H3 protein; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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