Document Detail


Beta-transforming growth factor is stored in human blood platelets as a latent high molecular weight complex.
MedLine Citation:
PMID:  3458465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human blood platelets, the richest known source of beta-transforming Growth Factor extractable under acid conditions, release in neutral extracts (pH 7.2) a latent form of this growth factor with an apparent molecular weight of 400 Kd. This latent form, poorly active on rat NRK-49F indicator cells in soft agar assays can be activated by exposure to acid pH or 8 molar urea. The acid activated beta-Transforming Growth Factor from neutral extracts elutes on Biogel P60, in 1 molar acetic acid, as a broad peak of apparent molecular weight 15-30 Kd, like when this factor is extracted from platelets by the usual acid-ethanol procedure. Moreover, beta-Transforming Growth Factor from both acid activated neutral extracts and from acid-ethanol extracts elutes on reverse phase at 30% acetonitrile. We suggest that beta-Transforming Growth Factor is stored in human blood platelets as a poorly active high molecular weight complex which may be dissociated and activated in appropriate in vivo microenvironments.
Authors:
R Pircher; P Jullien; D A Lawrence
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  136     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-06-06     Completed Date:  1986-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  30-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Platelets / metabolism*
Cell Line
Chromatography, Gel
Humans
Hydrogen-Ion Concentration
Macromolecular Substances
Molecular Weight
Peptides / blood*
Rats
Transforming Growth Factors
Urea / pharmacology
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 0/Peptides; 57-13-6/Urea; 76057-06-2/Transforming Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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