| Beta-transforming growth factor is stored in human blood platelets as a latent high molecular weight complex. | |
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MedLine Citation:
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PMID: 3458465 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human blood platelets, the richest known source of beta-transforming Growth Factor extractable under acid conditions, release in neutral extracts (pH 7.2) a latent form of this growth factor with an apparent molecular weight of 400 Kd. This latent form, poorly active on rat NRK-49F indicator cells in soft agar assays can be activated by exposure to acid pH or 8 molar urea. The acid activated beta-Transforming Growth Factor from neutral extracts elutes on Biogel P60, in 1 molar acetic acid, as a broad peak of apparent molecular weight 15-30 Kd, like when this factor is extracted from platelets by the usual acid-ethanol procedure. Moreover, beta-Transforming Growth Factor from both acid activated neutral extracts and from acid-ethanol extracts elutes on reverse phase at 30% acetonitrile. We suggest that beta-Transforming Growth Factor is stored in human blood platelets as a poorly active high molecular weight complex which may be dissociated and activated in appropriate in vivo microenvironments. |
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Authors:
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R Pircher; P Jullien; D A Lawrence |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 136 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-06-06 Completed Date: 1986-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 30-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Platelets / metabolism* Cell Line Chromatography, Gel Humans Hydrogen-Ion Concentration Macromolecular Substances Molecular Weight Peptides / blood* Rats Transforming Growth Factors Urea / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 0/Peptides; 57-13-6/Urea; 76057-06-2/Transforming Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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