Document Detail

Beta-sitosterol from psyllium seed husk (Plantago ovata Forsk) restores gap junctional intercellular communication in Ha-ras transfected rat liver cells.
MedLine Citation:
PMID:  15860444     Owner:  NLM     Status:  MEDLINE    
We purified compounds from the husks of psyllium seeds (Plantago ovata Forsk; desert Indian wheat), beginning with an ethanol extraction then followed by HP-20 and silica gel chromatography, which restored gap junctional intercellular communication (GJIC) in v-Ha-ras transfected rat liver epithelial WB-F344 cell line (WB-Ha-ras). GJIC was assessed by a scrape loading dye transfer assay. The active compound was identified as beta-sitosterol based on gas chromatography retention times and electron ionization mass spectroscopy (EI-MS) spectrum of authentic beta-sitosterol. Authentic beta-sitosterol restored GJIC in the tumorigenic WB-Ha-ras GJIC-deficient cells at a dose of 2.4 microM. In addition, a similar phytosterol, stigmasterol, also restored GJIC, albeit at a lower activity. beta-sitosterol and stigmasterol increased the level of connexin43 protein (Cx43) and restored phosphorylation of Cx43 to levels similar to the parental nontransfected cell line. We concluded that the restoration of intercellular communication in the GJIC-deficient, tumorigenic WB-Ha-ras cell line by the ethanol soluble fraction of psyllium seed husks is largely due to the presence of the phytosterol, beta-sitosterol. We discuss implications for dietary modulation of cancer by beta-sitosterol.
Yasushi Nakamura; Noriko Yoshikawa; Ikumi Hiroki; Kenji Sato; Kozo Ohtsuki; Chia-Cheng Chang; Brad L Upham; James E Trosko
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nutrition and cancer     Volume:  51     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  2005  
Date Detail:
Created Date:  2005-04-29     Completed Date:  2005-10-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-25     Citation Subset:  IM    
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Japan.
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MeSH Terms
Antilipemic Agents / chemistry,  pharmacology
Blotting, Western / methods
Cell Communication / drug effects*
Cell Transformation, Neoplastic / drug effects*
Cells, Cultured / metabolism
Gap Junctions / drug effects*
Gas Chromatography-Mass Spectrometry / methods
Genes, ras / physiology*
Hepatocytes / drug effects*,  pathology
Seeds / chemistry
Sitosterols / chemistry,  pharmacology*
Grant Support
Reg. No./Substance:
0/Antilipemic Agents; 0/Sitosterols; 5779-62-4/sitosterol; 8063-16-9/Psyllium

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