| Beta-oxidation of the geometric and positional isomers of octadecenoic acid by rat heart and liver mitochondria. | |
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MedLine Citation:
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PMID: 7284416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cis and trans isomers of delta 4 through delta 16 octadecenoic acid, all present in partially hydrogenated soybean oil, were compared as substrates for beta-oxidation by isolated rat heart and liver mitochondria. The fatty acids were converted to their coenzyme A esters and oxygen uptake rates measured polarographically in the presence of L-malate, L-carnitine, ADP, and optimum albumin. The cis isomers were catabolized in a similar pattern by heart and liver. The even-positioned cis isomers were oxidized significantly more slowly than adjacent odd-positioned isomers. Most odd-positioned cis isomers were oxidized as rapidly as oleoyl-CoA. The pattern of catabolism of the trans isomers, however, was different from the cis isomers. Liver mitochondria oxidized most even-positioned trans isomers significantly more rapidly than adjacent odd-positioned isomers. The same pattern was observed with heart mitochondria only for the trans isomers in which the double bond was located near the middle of the acyl chain. Heart mitochondria oxidized nearly all the trans isomers significantly more slowly than stearoyl-CoA; however, liver mitochondria oxidized the even-positioned trans isomers nearly as rapidly as stearoyl-CoA. Both heart and liver mitochondria oxidized the cis isomers, especially delta 9 and delta 11, significantly more rapidly than their respective trans isomers, with three notable exceptions: delta 8, delta 10, and delta 14. 3-Hydroxyacyl-CoA epimerase and delta 3-cis-delta-2-trans-enoyl-CoA isomerase account for most of the observed beta-oxidation patterns. An additional and more efficient pathway for the beta-oxidation of n-6 fatty acids is suggested. |
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Authors:
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L D Lawson; R T Holman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 665 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1981 Jul |
Date Detail:
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Created Date: 1981-12-15 Completed Date: 1981-12-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 60-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Isomerism Male Mitochondria, Heart / metabolism* Mitochondria, Liver / metabolism* Oleic Acid Oleic Acids / metabolism* Oxidation-Reduction Oxygen Consumption Rats |
| Grant Support | |
ID/Acronym/Agency:
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HL 08214/HL/NHLBI NIH HHS; HL 21513/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oleic Acids; 112-80-1/Oleic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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