| Beta-lapachone, a quinone isolated from Tabebuia avellanedae, induces apoptosis in HepG2 hepatoma cell line through induction of Bax and activation of caspase. | |
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MedLine Citation:
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PMID: 16822200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The DNA topoisomerase inhibitor beta-lapachone is a quinone obtained from the bark of the lapacho tree (Tabebuia avellanedae) in South America. It has been reported to possess a wide range of pharmacological properties, and is a promising cancer chemopreventive agent. In this study, the effects of beta-lapachone on the growth of the human hepatoma cell line HepG2 were investigated. The results showed that beta-lapachone inhibits the viability of HepG2 by inducing apoptosis, as evidenced by the formation of apoptotic bodies and DNA fragmentation. Reverse transcription-polymerase chain reaction and immunoblotting results indicated that treatments of cells with beta-lapachone resulted in down-regulation of anti-apoptotic Bcl-2 and Bcl-X(L) and up-regulation of pro-apoptotic Bax expression. beta-Lapachone-induced apoptosis was associated with a proteolytic activation of caspase-3 and -9 and degradation of poly(ADP-ribose) polymerase protein. However, beta-lapachone treatment did not affect the inhibitor of apoptosis proteins family and the Fas/FasL system. Taken together, our study indicated that beta-lapachone may have potential as a chemopreventive agent for liver cancer. |
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Authors:
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Hyun Joo Woo; Kun-Young Park; Chung-Ho Rhu; Won Ho Lee; Byung Tae Choi; Gi Young Kim; Yeong-Min Park; Yung Hyun Choi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medicinal food Volume: 9 ISSN: 1096-620X ISO Abbreviation: J Med Food Publication Date: 2006 |
Date Detail:
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Created Date: 2006-07-06 Completed Date: 2006-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9812512 Medline TA: J Med Food Country: United States |
Other Details:
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Languages: eng Pagination: 161-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD95
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analysis Apoptosis / drug effects* Carcinoma, Hepatocellular Caspase 3 Caspase 9 Caspases / metabolism* Cell Division / drug effects Cell Line, Tumor DNA Fragmentation Enzyme Activation / drug effects Fas Ligand Protein Flow Cytometry Gene Expression / drug effects Humans Inhibitor of Apoptosis Proteins / analysis Liver Neoplasms Membrane Glycoproteins / analysis Naphthoquinones / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics Reverse Transcriptase Polymerase Chain Reaction Tabebuia / chemistry* Tumor Necrosis Factors / analysis bcl-2-Associated X Protein / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Inhibitor of Apoptosis Proteins; 0/Membrane Glycoproteins; 0/Naphthoquinones; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Necrosis Factors; 0/bcl-2-Associated X Protein; 4707-32-8/beta-lapachone; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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