Document Detail


Beta-escin, a natural triterpenoid saponin from Chinese horse chestnut seeds, depresses HL-60 human leukaemia cell proliferation and induces apoptosis.
MedLine Citation:
PMID:  18718126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Beta-escin, a natural triterpenoid saponin isolated from the seed of the horse chestnut, is known to generate a wide variety of biochemical and pharmacological effects. The purpose of the present study was to examine the apoptotic and antiproliferative activity of beta-escin in HL-60 human acute myeloid leukaemia cells. Antiproliferative activity was examined by soft agar colony assay and the trypan blue exclusion method. Apoptotic activity was evaluated by morphological analysis, annexin V analysis, DNA fragmentation analysis and flow cytometry cell cycle analysis. The results showed that beta-escin caused a significant inhibition of HL-60 cell proliferation in a dose- and time-dependent manner. Morphological evidence of apoptosis, including vacuolization, apoptotic nuclei fragmentation and apoptotic body formation, was observed in cells treated with 30 microg mL(-1) of beta-escin for 24, 48 and 72 h. A significant increase in the population of annexin V+ and PI- cells (early apoptotic) among the total cells was observed in cells treated with beta-escin (30-50 microg mL(-1)) for 24 h (P<0.001). Typical DNA ladders, DNA with a unit length of about 180 bp, were detected in cells treated with beta-escin (30-50 microg mL(-1)) for 48 h by agarose gel electrophoresis. Flow cytometry cell cycle analysis revealed that beta-escin (30-50 microg mL(-1)) induced G1-S arrest and led to a significant accumulation of the sub-G1 population in HL-60 cells (P<0.05). Taken together, the results demonstrate that beta-escin is a potent natural inhibitor of cell proliferation and inducer of apoptosis in HL-60 acute myeloid leukaemia cells. The results indicate that beta-escin may be a useful candidate agent for exploring potential antileukaemic drugs.
Authors:
Yang P Niu; Li M Wu; Yan L Jiang; Wen X Wang; Lian D Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  60     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-22     Completed Date:  2008-12-11     Revised Date:  2009-08-20    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1213-20     Citation Subset:  IM    
Affiliation:
Institute of Chinese Herbal Medicine, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China. niuyangping@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Aesculus / chemistry*
Antineoplastic Agents, Phytogenic / administration & dosage,  isolation & purification,  pharmacology*
Apoptosis / drug effects*
Cell Cycle / drug effects
Cell Proliferation / drug effects
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Escin / administration & dosage,  isolation & purification,  pharmacology*
Flow Cytometry
G1 Phase / drug effects
HL-60 Cells
Humans
Leukemia, Promyelocytic, Acute / drug therapy
S Phase / drug effects
Seeds
Time Factors
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 6805-41-0/Escin
Comments/Corrections
Retraction In:
Jones DS. J Pharm Pharmacol. 2009 May;61(5):685   [PMID:  19406009 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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