| Beta-escin, a natural triterpenoid saponin from Chinese horse chestnut seeds, depresses HL-60 human leukaemia cell proliferation and induces apoptosis. | |
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MedLine Citation:
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PMID: 18718126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Beta-escin, a natural triterpenoid saponin isolated from the seed of the horse chestnut, is known to generate a wide variety of biochemical and pharmacological effects. The purpose of the present study was to examine the apoptotic and antiproliferative activity of beta-escin in HL-60 human acute myeloid leukaemia cells. Antiproliferative activity was examined by soft agar colony assay and the trypan blue exclusion method. Apoptotic activity was evaluated by morphological analysis, annexin V analysis, DNA fragmentation analysis and flow cytometry cell cycle analysis. The results showed that beta-escin caused a significant inhibition of HL-60 cell proliferation in a dose- and time-dependent manner. Morphological evidence of apoptosis, including vacuolization, apoptotic nuclei fragmentation and apoptotic body formation, was observed in cells treated with 30 microg mL(-1) of beta-escin for 24, 48 and 72 h. A significant increase in the population of annexin V+ and PI- cells (early apoptotic) among the total cells was observed in cells treated with beta-escin (30-50 microg mL(-1)) for 24 h (P<0.001). Typical DNA ladders, DNA with a unit length of about 180 bp, were detected in cells treated with beta-escin (30-50 microg mL(-1)) for 48 h by agarose gel electrophoresis. Flow cytometry cell cycle analysis revealed that beta-escin (30-50 microg mL(-1)) induced G1-S arrest and led to a significant accumulation of the sub-G1 population in HL-60 cells (P<0.05). Taken together, the results demonstrate that beta-escin is a potent natural inhibitor of cell proliferation and inducer of apoptosis in HL-60 acute myeloid leukaemia cells. The results indicate that beta-escin may be a useful candidate agent for exploring potential antileukaemic drugs. |
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Authors:
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Yang P Niu; Li M Wu; Yan L Jiang; Wen X Wang; Lian D Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 60 ISSN: 0022-3573 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-22 Completed Date: 2008-12-11 Revised Date: 2009-08-20 |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 1213-20 Citation Subset: IM |
Affiliation:
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Institute of Chinese Herbal Medicine, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China. niuyangping@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aesculus
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chemistry* Antineoplastic Agents, Phytogenic / administration & dosage, isolation & purification, pharmacology* Apoptosis / drug effects* Cell Cycle / drug effects Cell Proliferation / drug effects DNA Fragmentation / drug effects Dose-Response Relationship, Drug Escin / administration & dosage, isolation & purification, pharmacology* Flow Cytometry G1 Phase / drug effects HL-60 Cells Humans Leukemia, Promyelocytic, Acute / drug therapy S Phase / drug effects Seeds Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 6805-41-0/Escin |
| Comments/Corrections | |
Retraction In:
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Jones DS. J Pharm Pharmacol. 2009 May;61(5):685
[PMID:
19406009
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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