Document Detail

Beta cell neogenesis from ducts and phenotypic conversion of residual islet cells in the adult pancreas of glucose intolerant mice induced by selective alloxan perfusion.
MedLine Citation:
PMID:  12507275     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to clarify the pattern of beta cell neogenesis in the alloxan-perfused, beta cells-depleted segment of glucose intolerant mice induced by selective alloxan perfusion. First, duct cells proliferated in the perfused segment, then cells co-expressing multiple islet hormones and transcription factors such as PDX-1, Nkx2.2, Isl1, and Pax6 were observed in duct cells, and newly formed islet-like cell clusters (ICCs) containing beta cells were recognized. In residual beta cell-depleted islets, glucagon or somatostatin and PDX-1 double-positive immature endocrine cells were recognized. Glucagon or somatostatin, insulin and PDX-1 triple-positive cells then appeared and these cells appeared to undergo terminal differentiation into beta cells. In conclusion, we demonstrated at least two different processes of beta cell neogenesis, i.e., formation of new ICCs from ductal epithelium and redifferentiation of residual non-beta islet cells in this model. In addition, transcription factors that appear in the processes of endocrine cell development may also play essential roles during beta cell neogenesis from duct cells.
Ming Li; Jun-ichiro Miyagawa; Koji Yamamoto; Makoto Moriwaki; Akihisa Imagawa; Hiromi Iwahashi; Kazuya Yamagata; Yoshihiro Tochino; Toshiaki Hanafusa; Yuji Matsuzawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine journal     Volume:  49     ISSN:  0918-8959     ISO Abbreviation:  Endocr. J.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-12-31     Completed Date:  2003-05-27     Revised Date:  2011-06-01    
Medline Journal Info:
Nlm Unique ID:  9313485     Medline TA:  Endocr J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  561-72     Citation Subset:  IM    
Department of Internal Medicine and Molecular Science, Graduate School of Medicine, B5, Osaka University, 2-2 Yamadaoka, Suita 565-0871, Japan.
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MeSH Terms
Alloxan / administration & dosage*
Cell Differentiation*
Cell Division
Glucagon / analysis
Glucose Intolerance / chemically induced,  pathology*
Homeodomain Proteins*
Insulin / analysis
Islets of Langerhans / chemistry,  pathology*
Mice, Inbred ICR
Microscopy, Electron
Pancreatic Ducts / chemistry,  pathology*
Somatostatin / analysis
Trans-Activators / analysis
Reg. No./Substance:
0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein; 11061-68-0/Insulin; 50-71-5/Alloxan; 51110-01-1/Somatostatin; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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