Document Detail


Beta cell function during rapamycin monotherapy in long-term type 1 diabetes.
MedLine Citation:
PMID:  21046356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS/HYPOTHESIS: Type 1 diabetes is considered non-reversible at end-stage disease when there is no measurable insulin production. However, there are indications that insulin-producing beta cells could be present or return if autoimmunity could be controlled. We therefore sought to determine whether immunosuppression therapy can reinstate beta cell function in patients with long-term type 1 diabetes.
METHODS: We examined pancreatic beta cell function in 22 patients with long-term type 1 diabetes (median disease duration 27 years), who had been receiving rapamycin monotherapy (0.1 mg/kg; target trough levels 8-10 ng/ml; 26-314 days) as pre-conditioning for islet transplantation. As control, beta cell function was measured in 14 patients (median disease duration 17 years) who were waiting for an islet transplant without rapamycin pre-conditioning.
RESULTS: Fasting C-peptide increased from <0.03 nmol/l (0.0066 nmol/l, interquartile range [IQR] 0.0003-0.023) at baseline to 0.039 nmol/l (IQR 0.0066-0.096) at end of rapamycin monotherapy (p < 0.005). In 12 patients, fasting C-peptide increased to >0.076 nmol/l (C-peptide responders). Exogenous insulin requirement decreased from 0.64 U/kg daily (IQR 0.56-0.72) to 0.57 U/kg (IQR 0.45-0.70; p = 0.01), but this reduction was significant only in the 12C-peptide-responsive patients. Rapamycin monotherapy was also associated with a decrease in insulin antibody titre (median decrease 110 to 35.9 U/ml; p < 0.001) and fasting serum proinsulin (median decrease 0.51 to 0.28 pmol/l; p = 0.001). All variables remained unchanged in the 14 control patients.
CONCLUSIONS/INTERPRETATION: Therapies to reinstate beta cell function may be applicable to patients with long-term C-peptide-negative type 1 diabetes.
TRIAL REGISTRATION: ClinicalTrial.gov NCT01060605.
Authors:
L Piemonti; P Maffi; L Monti; V Lampasona; G Perseghin; P Magistretti; A Secchi; E Bonifacio
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-03
Journal Detail:
Title:  Diabetologia     Volume:  54     ISSN:  1432-0428     ISO Abbreviation:  Diabetologia     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-07     Completed Date:  2011-05-02     Revised Date:  2011-11-24    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  433-9     Citation Subset:  IM    
Affiliation:
Diabetes Research Institute (HSR-DRI), San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy. piemonti.lorenzo@hsr.it
Data Bank Information
Bank Name/Acc. No.:
ClinicalTrials.gov/NCT01060605
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MeSH Terms
Descriptor/Qualifier:
Adult
Diabetes Mellitus, Type 1 / blood,  drug therapy*,  physiopathology*
Female
Humans
Immunosuppressive Agents / therapeutic use*
Insulin-Secreting Cells / physiology*
Male
Middle Aged
Proinsulin / blood
Sirolimus / therapeutic use*
Grant Support
ID/Acronym/Agency:
JT01Y01//Telethon
Chemical
Reg. No./Substance:
0/Immunosuppressive Agents; 53123-88-9/Sirolimus; 9035-68-1/Proinsulin
Comments/Corrections
Comment In:
Nat Rev Endocrinol. 2011 Feb;7(2):62   [PMID:  21332092 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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