| Beta cell differentiation during early human pancreas development. | |
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MedLine Citation:
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PMID: 15072563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c., human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy. |
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Authors:
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K Piper; S Brickwood; L W Turnpenny; I T Cameron; S G Ball; D I Wilson; N A Hanley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of endocrinology Volume: 181 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-04-09 Completed Date: 2004-06-08 Revised Date: 2011-06-01 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 11-23 Citation Subset: IM |
Affiliation:
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Division of Human Genetics, University of Southampton, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis Cell Differentiation Cell Nucleus / chemistry Cells, Cultured Cytoplasm / chemistry Embryonic and Fetal Development / physiology Epithelial Cells / chemistry, cytology* Gestational Age Glucagon / analysis Homeodomain Proteins* Humans Immunohistochemistry / methods Insulin / analysis Islets of Langerhans / chemistry, cytology, embryology* Keratins / analysis Mice Trans-Activators / analysis |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein; 11061-68-0/Insulin; 68238-35-7/Keratins; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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