| Beta-catenin regulation during the cell cycle: implications in G2/M and apoptosis. | |
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MedLine Citation:
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PMID: 12857869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Beta-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. Beta-catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems beta-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of beta-catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous beta-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase-synchronized epithelial cells. Beta-catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of beta-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not beta-catenin, is detected in M phase. Interestingly, overexpression of a stable form of beta-catenin, or inhibition of endogenous beta-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for beta-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes. |
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Authors:
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David Olmeda; Susanna Castel; Senén Vilaró; Amparo Cano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-04-04 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 14 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-07-14 Completed Date: 2004-04-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2844-60 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, 28029 Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenomatous Polyposis Coli Protein
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metabolism* Animals Apoptosis / physiology* Cell Adhesion / physiology Cell Nucleus / metabolism Cells, Cultured Centrosome / metabolism Cytoskeletal Proteins / metabolism* Dogs Flow Cytometry G2 Phase / physiology* Humans Mice Microscopy, Confocal Mitosis / physiology* Trans-Activators / metabolism* beta Catenin |
| Chemical | |
Reg. No./Substance:
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0/Adenomatous Polyposis Coli Protein; 0/CTNNB1 protein, human; 0/Catnb protein, mouse; 0/Cytoskeletal Proteins; 0/Trans-Activators; 0/beta Catenin |
| Comments/Corrections | |
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