Document Detail

Beta-catenin can bind directly to CRM1 independently of adenomatous polyposis coli, which affects its nuclear localization and LEF-1/beta-catenin-dependent gene expression.
MedLine Citation:
PMID:  18262809     Owner:  NLM     Status:  MEDLINE    
Nuclear beta-catenin affects the developmental process and progression of tumors. However, the precise mechanism for the nuclear export of beta-catenin is not completely understood. We found that beta-catenin can bind directly to CRM1 through its central armadillo (ARM) repeats region, independently of the adenomatous polyposis coli (APC) protein. CRM1 overexpression transports nuclear beta-catenin into the cytoplasm and decreases LEF-1/beta-catenin-dependent transcriptional activity, which is also affected by the co-overexpression of E-cadherin. CRM1 competed with E-cadherin and LEF-1 for binding to beta-catenin. beta-catenin could interact directly with APC through its essential sequences between amino acids 342 and 350. The site-directed beta-catenin mutant (NES2(-)), which could interact with CRM1, but not with APC, still retained its ability to export from the nucleus and its transactivational activity. This suggests that CRM1 can function as an efficient nuclear exporter for beta-catenin independently of APC. These results strongly suggest that the CRM1-mediated pathway is involved in the efficient transport of nuclear beta-catenin in the nucleus of cells.
Hyunkyoung Ki; Minsoo Oh; Su Wol Chung; Kwonseop Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-09
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-02     Completed Date:  2008-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  394-400     Citation Subset:  IM    
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, 300 Yongbong-dong, Gwangju, South Korea.
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MeSH Terms
Active Transport, Cell Nucleus
Adenomatous Polyposis Coli Protein / metabolism
Amino Acid Sequence
Binding, Competitive
Cadherins / metabolism
Cell Nucleus / metabolism*
Gene Expression Regulation*
Karyopherins / metabolism*
Lymphoid Enhancer-Binding Factor 1 / metabolism*
Models, Biological
Molecular Sequence Data
NIH 3T3 Cells
Nuclear Export Signals
Protein Binding
Receptors, Cytoplasmic and Nuclear / metabolism*
Repetitive Sequences, Amino Acid
beta Catenin / chemistry,  metabolism*
Reg. No./Substance:
0/Adenomatous Polyposis Coli Protein; 0/Cadherins; 0/Karyopherins; 0/Lymphoid Enhancer-Binding Factor 1; 0/Nuclear Export Signals; 0/Receptors, Cytoplasmic and Nuclear; 0/beta Catenin; 0/exportin 1 protein

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