| Beta-alanine protection against hypoxic liver injury in the rat. | |
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MedLine Citation:
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PMID: 12009428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Liver hypoxia still represents an important cause of liver injury during shock and liver transplantation. We have investigated the protective effects of beta-alanine against hypoxic injury using isolated perfused rat livers and isolated rat hepatocyte suspensions. Perfusion with hypoxic Krebs-Henseleit buffer increased liver weight and caused a progressive release of lactate dehydrogenase (LDH) in the effluent perfusate. The addition of 5 mmol/l beta-alanine to the perfusion buffer completely prevented both weight increase and LDH leakage. These findings were confirmed by histological examinations showing that beta-alanine blocked the staining by trypan blue of either liver parenchymal and sinusoidal cells. Studies performed in isolated hepatocytes revealed that beta-alanine exerted its protective effects by interfering with Na+ accumulation induced by hypoxia. The addition of gamma-amino-butyric acid, which interfered with beta-alanine uptake by the hepatocytes or of Na+/H+ ionophore monensin, reverted beta-alanine protection in either hepatocyte suspensions or isolated perfused livers. We also observed that liver receiving beta-alanine were also protected against LDH leakage and weight increase caused by the perfusion with an hyposmotic (205 mosm) hypoxic buffer obtained by decreasing NaCl content from 118 to 60 mmol/l. This latter effect was not reverted by blocking K+ efflux from hepatocyte with BaCl(2) (1mmol/l). Altogether these results indicated that beta-alanine protected against hypoxic liver injury by preventing Na+ overload and by increasing liver resistance to osmotic stress consequent to the impairment of ion homeostasis during hypoxia. |
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Authors:
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Mariapia Vairetti; Rita Carini; Maria Grazia De Cesaris; Roberta Splendore; Plinio Richelmi; Francantonio Bertè; Emanuele Albano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1587 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-05-15 Completed Date: 2002-08-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 83-91 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of Pavia, Piazza Botta 10, 27100, Italy. vairetti@botta.unipv.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Hypoxia Cell Survival Cells, Cultured Cytoprotection* Dose-Response Relationship, Drug Hypotonic Solutions Liver / drug effects*, pathology Perfusion Rats Trypan Blue beta-Alanine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Hypotonic Solutions; 107-95-9/beta-Alanine; 72-57-1/Trypan Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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