Document Detail

Beta adrenergic receptors in human cavernous tissue.
MedLine Citation:
PMID:  2858597     Owner:  NLM     Status:  MEDLINE    
Beta adrenergic receptor binding was performed with 125I iodocyanopindolol on human cavernous tissue membrane fractions from normal tissue and transsexual procedures obtained postoperatively, as well as from postmortem sources. Isotherm binding studies on normal fresh tissues indicated that the receptor density was 9.1 fmoles/mg. with a KD of 23 pM. Tissue stored at room temperature for 4 to 6 hours, then at 4C in saline solution for 19 to 20 hours before freezing showed no significant changes in receptor density or affinity, and provided evidence for the stability of postmortem tissue obtained within the same time period. Beta receptor density of 2 cavernous preparations from transsexual procedures was not significantly different from normal control tissues, and showed that high concentrations of estrogen received by these patients had no effect on beta adrenergic receptor density. Displacement of 125iodocyanopindolol by 5 beta adrenergic agents demonstrated that 1-propranolol had the greatest affinity followed by ICI 118,551, zinterol, metoprolol and practolol. When the results of these displacement studies were subjected to Scatfit, non-linear regression line analysis, a single binding site was described. Based on the relative potency of the selective beta adrenergic agents it appears that these receptors were of the beta 2 subtype.
C B Dhabuwala; C V Ramakrishna; G F Anderson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of urology     Volume:  133     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-05-02     Completed Date:  1985-05-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  721-3     Citation Subset:  AIM; IM    
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MeSH Terms
Adrenergic beta-Agonists / pharmacology
Adrenergic beta-Antagonists / pharmacology
Binding Sites
Ethanolamines / pharmacology
Iodine Radioisotopes / diagnostic use
Metoprolol / pharmacology
Penis / innervation*
Pindolol / analogs & derivatives,  diagnostic use
Practolol / pharmacology
Propanolamines / pharmacology
Propranolol / pharmacology
Receptors, Adrenergic, beta / analysis*,  drug effects,  metabolism
Grant Support
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Adrenergic beta-Antagonists; 0/Ethanolamines; 0/Iodine Radioisotopes; 0/Propanolamines; 0/Receptors, Adrenergic, beta; 13523-86-9/Pindolol; 37000-20-7/zinterol; 37350-58-6/Metoprolol; 525-66-6/Propranolol; 6673-35-4/Practolol; 72795-19-8/ICI 118551; 83498-72-0/Iodocyanopindolol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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