Document Detail

Beta Cells Are Not Generated in Pancreatic Duct Ligation Induced Injury in Adult Mice.
MedLine Citation:
PMID:  23349489     Owner:  NLM     Status:  Publisher    
The existence of adult β-cell progenitors remains the most controversial developmental biology topic in diabetes research. It has been reported that β-cell progenitors can be activated by ductal ligation induced injury of adult mouse pancreas, which apparently act in a cell autonomous manner to double the functional β-cell mass within a week by differentiation and proliferation. Here, we demonstrate that pancreatic ductal ligation (PDL) does not activate progenitors to contribute to β-cell mass expansion. Rather, PDL stimulates massive pancreatic injury, which alters pancreatic composition and thus complicates accurate measurement of β-cell content via traditional morphometry methodologies that superficially sample the pancreas. To overcome this potential bias we quantified β-cells from the entire pancreas and observed that β-cell mass and insulin content are totally unchanged by PDL-induced injury. Lineage tracing studies using sequential administration of thymidine analogues, rat insulin 2 promoter driven cre-lox, and low-frequency ubiquitous cre-lox reveal that PDL does not convert progenitors to the β-cell lineage. Thus, we conclude that β-cells are not generated in injured adult mouse pancreas.
Matthew M Rankin; Christopher J Wilbur; Kimberly Rak; Emily J Shields; Anne Granger; Jake A Kushner
Related Documents :
24142249 - A replication-inhibited unsegregated nucleoid at mid-cell blocks z-ring formation and c...
24604009 - Isolinderalactone inhibits proliferation of a549 human non‑small cell lung cancer cel...
23069719 - Nuclear glutathione.
22703589 - Satellite cell heterogeneity revealed by g-tool, an open algorithm to quantify myogenes...
24386239 - Cell cycle-dependent rho gtpase activity dynamically regulates cancer cell motility and...
23568979 - Evaluation of apoptotic activity of new condensed pyrazole derivatives.
22011009 - Resveratrol enhances the cytotoxic profile of docetaxel and doxorubicin in solid tumour...
9453249 - V3 loop of human immunodeficiency virus type 1 reduces cyclin e expression and induces ...
12843439 - Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Diabetes     Volume:  -     ISSN:  1939-327X     ISO Abbreviation:  Diabetes     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Division of Endocrinology and Diabetes, Philadelphia, Pennsylvania, United States.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The type 2 diabetes associated gene Ide is required for insulin secretion and suppression of ?-synuc...
Next Document:  Enhanced NF-?B activity impairs vascular function through PARP-1-, SP-1-, and COX-2-dependent mechan...