Document Detail


Beta Cells Are Not Generated in Pancreatic Duct Ligation Induced Injury in Adult Mice.
MedLine Citation:
PMID:  23349489     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The existence of adult β-cell progenitors remains the most controversial developmental biology topic in diabetes research. It has been reported that β-cell progenitors can be activated by ductal ligation induced injury of adult mouse pancreas, which apparently act in a cell autonomous manner to double the functional β-cell mass within a week by differentiation and proliferation. Here, we demonstrate that pancreatic ductal ligation (PDL) does not activate progenitors to contribute to β-cell mass expansion. Rather, PDL stimulates massive pancreatic injury, which alters pancreatic composition and thus complicates accurate measurement of β-cell content via traditional morphometry methodologies that superficially sample the pancreas. To overcome this potential bias we quantified β-cells from the entire pancreas and observed that β-cell mass and insulin content are totally unchanged by PDL-induced injury. Lineage tracing studies using sequential administration of thymidine analogues, rat insulin 2 promoter driven cre-lox, and low-frequency ubiquitous cre-lox reveal that PDL does not convert progenitors to the β-cell lineage. Thus, we conclude that β-cells are not generated in injured adult mouse pancreas.
Authors:
Matthew M Rankin; Christopher J Wilbur; Kimberly Rak; Emily J Shields; Anne Granger; Jake A Kushner
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Diabetes     Volume:  -     ISSN:  1939-327X     ISO Abbreviation:  Diabetes     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Division of Endocrinology and Diabetes, Philadelphia, Pennsylvania, United States.
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