Document Detail


Beta cell mass regulation in the rat pancreas through glucocorticoids and thyroid hormones.
MedLine Citation:
PMID:  20683219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To compare the effects of glucocorticoids and thyroid hormones on the regulation of the beta cell mass in the pancreas, the rats were treated and analyzed for cell cycle changes in islet and duct cells as a source for beta cell neogenesis.
METHODS: Different rat pancreases were morphometrically analyzed after immunohistochemical staining for markers of proliferation and apoptosis.
RESULTS: Hydrocortisone increased the beta cell mass of rat pancreases through an increase of proliferation. This effect was counteracted by an increase of apoptosis. In contrast, thyroxine decreased the beta cell mass through an increase of apoptosis. This effect was counteracted by an increased rate of proliferation. Combined treatment with both hormones nullified the antagonistic effects on proliferation, apoptosis, and beta cell mass, thereby contributing to the maintenance of a stable total beta cell volume of the pancreas.
CONCLUSIONS: Hydrocortisone and thyroxine induced analogous changes in pancreatic duct cells, which represent a crucial pool for new beta cells through neogenesis. This may explain the positive effects of glucocorticoids in the immunosuppressive therapy regimen after whole pancreas transplantation upon long-term insulin independence, which is not achievable with isolated islets because of the loss of duct cells during the islet process before transplantation.
Authors:
Anne Jörns; Christoph Sennholz; Ortwin Naujok; Sigurd Lenzen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pancreas     Volume:  39     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-15     Completed Date:  2011-03-02     Revised Date:  2011-06-01    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1167-72     Citation Subset:  IM    
Affiliation:
Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany. Joerns.Anne@mh-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Bromodeoxyuridine / metabolism
Cell Count
Cell Proliferation / drug effects*
Glucocorticoids / pharmacology*
Homeodomain Proteins / metabolism
Hydrocortisone / pharmacology
Immunohistochemistry
In Situ Nick-End Labeling
Insulin-Secreting Cells / cytology,  drug effects*,  metabolism
Pancreatic Ducts / cytology,  drug effects,  metabolism
Rats
Rats, Inbred Lew
Thyroid Hormones / pharmacology*
Thyroxine / pharmacology
Trans-Activators / metabolism
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Homeodomain Proteins; 0/Thyroid Hormones; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein; 50-23-7/Hydrocortisone; 59-14-3/Bromodeoxyuridine; 7488-70-2/Thyroxine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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