| Beta-Amyloid peptide and amyloid precursor proteins in olfactory mucosa of patients with Alzheimer's disease, Parkinson's disease, and Down syndrome. | |
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MedLine Citation:
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PMID: 7639477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dystrophic neurites are present in olfactory epithelium (OE) of patients with Alzheimer's disease (AD), Parkinson's disease (PD), and Down syndrome (DS) and occasionally in normal individuals. Cultured olfactory neuroblasts from AD patients generate carboxy terminal amyloid precursor protein (APP) fragments that contain beta-amyloid (A beta), but it is not known if deposits of A beta and/or APP fragments occur in the OE of individuals with or without AD, PD, or DS. To determine if A beta accumulates in the OE in situ, we probed postmortem samples of olfactory mucosa from patients with AD, PD, and AD (PD/AD), and DS and AD (DS/AD), as well as from controls, using polyclonal and monoclonal antibodies to A beta and flanking sequences in APPs. Samples of OE also were examined by thioflavin-S and electron microscopy. Labeling of A beta was observed in 10 of 12 AD cases, 2 of 3 PD/AD cases, 3 of 4 DS/AD cases, 3 of 10 adult controls, and 4 of 6 fetal cases. The A beta staining was seen in the basal third of the OE, in axons projecting through the lamina propria, and in metaplastic respiratory epithelium within the OE. Antibodies to other APP domains stained the OE of patients and controls. Thioflavin-S staining was present in the basal third of the OE of 8 of 9 AD patients and several PD/AD and DS/AD patients, but only in rare cells of 3 controls. Electron microscopy did not reveal amyloid fibrils in the OE.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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P B Crino; J A Martin; W D Hill; B Greenberg; V M Lee; J Q Trojanowski |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Annals of otology, rhinology, and laryngology Volume: 104 ISSN: 0003-4894 ISO Abbreviation: Ann. Otol. Rhinol. Laryngol. Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1995-09-13 Completed Date: 1995-09-13 Revised Date: 2006-11-28 |
Medline Journal Info:
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Nlm Unique ID: 0407300 Medline TA: Ann Otol Rhinol Laryngol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 655-61 Citation Subset: AIM; IM |
Affiliation:
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Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Alzheimer Disease / metabolism, pathology* Amyloid / analysis* Amyloid beta-Protein / analysis* Case-Control Studies Down Syndrome / metabolism, pathology* Fetus / pathology Humans Immunohistochemistry Infant Infant, Newborn Middle Aged Nasal Mucosa / chemistry* Parkinson Disease / metabolism, pathology* Prions Protein Precursors / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amyloid; 0/Amyloid beta-Protein; 0/PRNP protein, human; 0/Prions; 0/Protein Precursors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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