Document Detail


Beta-2-microglobulin haplotypes in U.S. beef cattle and association with failure of passive transfer in newborn calves.
MedLine Citation:
PMID:  15014972     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Failure of passive transfer (FPT) is a condition in which neonates do not acquire protective serum levels of maternal antibodies. A principal component of antibody transport is the neonatal receptor for the Fc portion of immunoglobulin, a heterodimer of a MHC-1 alpha-chain homolog ( FCGRT) and beta-2-microglobulin ( B2M). Previously, two FCGRT haplotypes were associated with differences in immunoglobulin G (IgG) passive transfer in cattle (Laegreid et al. (2002) Mamm Genome 13, 704-710). The present study had two objectives: first, to characterize the B2M haplotype structure in a diverse group of U.S. beef cattle, and second, to evaluate those haplotypes for association with either high or low serum IgG levels in newborn calves. Twelve single nucleotide polymorphisms (SNPs), assorted into eight haplotypes, were identified by sequencing regions of B2M exons II and IV in a multi-breed panel of 96 beef cattle. Calves homozygous for one of the eight haplotypes ( B2M 2,2) were at increased risk of FPT (odds ratio = 10.60, CI(95%) 2.07-54.24, p = 0.005). These results indicate that this haplotype is in linkage disequilibrium with genetic risk factors affecting passive transfer of IgG in beef calves, an important determinant of neonatal calf morbidity and mortality.
Authors:
Michael L Clawson; Michael P Heaton; Carol G Chitko-McKown; James M Fox; Timothy P L Smith; Warren M Snelling; John W Keele; William W Laegreid
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Mammalian genome : official journal of the International Mammalian Genome Society     Volume:  15     ISSN:  0938-8990     ISO Abbreviation:  Mamm. Genome     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-11     Completed Date:  2004-10-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9100916     Medline TA:  Mamm Genome     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-36     Citation Subset:  IM    
Affiliation:
United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center (MARC), Spur 18D, P.O. Box 166, Clay Center, Nebraska 68933, USA. clawson@email.marc.usda.gov
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MeSH Terms
Descriptor/Qualifier:
Animal Husbandry / methods
Animals
Animals, Newborn / immunology*
Base Sequence
Cattle / genetics*
Chromosome Mapping
DNA Primers
Genetic Variation*
Genotype
Haplotypes / genetics*
Histocompatibility Antigens Class I
Immunity, Maternally-Acquired
Immunoglobulin G / blood*
Linkage Disequilibrium
Molecular Sequence Data
Polymorphism, Single Nucleotide
Receptors, Fc / genetics*
Sequence Analysis, DNA
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
United States
beta 2-Microglobulin / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Fc receptor, neonatal; 0/Histocompatibility Antigens Class I; 0/Immunoglobulin G; 0/Receptors, Fc; 0/beta 2-Microglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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