| Beta-1,3:1,4-glucan synthase activity in rice seedlings under water. | |
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MedLine Citation:
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PMID: 18487614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: The metabolism of beta-1,3 : 1,4-glucan regulates the mechanical properties of cell walls, and thereby changes the elongation growth of Poaceae plants. A previous study has shown that elongation growth of rice coleoptiles under water is enhanced by increased activity of beta-1,3 : 1,4-glucan hydrolases; however, the involvement of beta-1,3 : 1,4-glucan synthase activity in elongation growth under water has not yet been clarified. METHODS: The beta-1,3 : 1,4-glucan synthase activity in a microsomal fraction prepared from rice seedlings grown under water was compared with that from control seedlings grown in air. The change under water in the relative expression level of CslF6, a major isoform of the beta-1,3 : 1,4-glucan synthase genes, was examined by quantitative reverse-transcriptase PCR. KEY RESULTS: The level of beta-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40 % of that of the control seedlings and was accompanied by a significant reduction in the amount of beta-1,3 : 1,4-glucan in the cell walls. Under water, the expression of CslF6 was reduced to less than 20 % of the unsubmerged control. Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water, correlating with suppression of the submergence-induced elongation growth of coleoptiles. CONCLUSIONS: Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of beta-1,3 : 1,4-glucan synthase activity. Together with the increased activity of beta-1,3 : 1,4-glucan hydrolases, the decreased activity of beta-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of beta-1,3 : 1,4-glucan in the cell walls under water. The suppression of beta-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion. |
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Authors:
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Tomoya Kimpara; Tsutomu Aohara; Kouichi Soga; Kazuyuki Wakabayashi; Takayuki Hoson; Yoichi Tsumuraya; Toshihisa Kotake |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-16 |
Journal Detail:
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Title: Annals of botany Volume: 102 ISSN: 1095-8290 ISO Abbreviation: Ann. Bot. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-16 Completed Date: 2008-08-14 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 0372347 Medline TA: Ann Bot Country: England |
Other Details:
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Languages: eng Pagination: 221-6 Citation Subset: IM |
Affiliation:
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Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama 338-8570, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Wall
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chemistry Cotyledon / enzymology, growth & development Down-Regulation Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glucosyltransferases / metabolism* Oryza sativa / enzymology*, growth & development* Plant Proteins / metabolism RNA, Messenger / metabolism RNA, Plant / metabolism Seedling / enzymology*, growth & development* Water beta-Glucans / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Plant; 0/beta-Glucans; 55965-23-6/beta-glucan, (1-3)(1-4)-; 7732-18-5/Water; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/glucan synthase |
| Comments/Corrections | |
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