Document Detail


Beta-1,3:1,4-glucan synthase activity in rice seedlings under water.
MedLine Citation:
PMID:  18487614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: The metabolism of beta-1,3 : 1,4-glucan regulates the mechanical properties of cell walls, and thereby changes the elongation growth of Poaceae plants. A previous study has shown that elongation growth of rice coleoptiles under water is enhanced by increased activity of beta-1,3 : 1,4-glucan hydrolases; however, the involvement of beta-1,3 : 1,4-glucan synthase activity in elongation growth under water has not yet been clarified. METHODS: The beta-1,3 : 1,4-glucan synthase activity in a microsomal fraction prepared from rice seedlings grown under water was compared with that from control seedlings grown in air. The change under water in the relative expression level of CslF6, a major isoform of the beta-1,3 : 1,4-glucan synthase genes, was examined by quantitative reverse-transcriptase PCR. KEY RESULTS: The level of beta-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40 % of that of the control seedlings and was accompanied by a significant reduction in the amount of beta-1,3 : 1,4-glucan in the cell walls. Under water, the expression of CslF6 was reduced to less than 20 % of the unsubmerged control. Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water, correlating with suppression of the submergence-induced elongation growth of coleoptiles. CONCLUSIONS: Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of beta-1,3 : 1,4-glucan synthase activity. Together with the increased activity of beta-1,3 : 1,4-glucan hydrolases, the decreased activity of beta-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of beta-1,3 : 1,4-glucan in the cell walls under water. The suppression of beta-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion.
Authors:
Tomoya Kimpara; Tsutomu Aohara; Kouichi Soga; Kazuyuki Wakabayashi; Takayuki Hoson; Yoichi Tsumuraya; Toshihisa Kotake
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-16
Journal Detail:
Title:  Annals of botany     Volume:  102     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-16     Completed Date:  2008-08-14     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
Affiliation:
Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama 338-8570, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / chemistry
Cotyledon / enzymology,  growth & development
Down-Regulation
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Glucosyltransferases / metabolism*
Oryza sativa / enzymology*,  growth & development*
Plant Proteins / metabolism
RNA, Messenger / metabolism
RNA, Plant / metabolism
Seedling / enzymology*,  growth & development*
Water
beta-Glucans / metabolism
Chemical
Reg. No./Substance:
0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Plant; 0/beta-Glucans; 55965-23-6/beta-glucan, (1-3)(1-4)-; 7732-18-5/Water; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/glucan synthase
Comments/Corrections

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