| Berberine inhibits p53-dependent cell growth through induction of apoptosis of prostate cancer cells. | |
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MedLine Citation:
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PMID: 19360335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Berberine has anti-tumor properties in some cancer cells including prostate cancer, but the exact mechanisms and in vivo effects are unclear. We investigated anti-cancer activity of berberine in vitro and in vivo, and possible mechanisms in prostate cancer cells. Berberine treatment inhibited cell cancer growth in a concentration (0-50 microM) and time- (0-48 h) dependent manner without any growth inhibition in normal human prostate epithelial PWR-1E cells. However, the p53 expressing LNCaP cells were more susceptible against berberine than the p53 lacking PC-3 cells. The cell arrest in G0/G1 phase, apoptotic cell death and the expression of apoptotic cell death proteins Bax and caspase-3 was much higher in berberine-treated LNCaP cells than those in PC-3 cells. Exploration of p53 siRNA or pifithrin-alpha, a p53 inhibitor to the LNCaP cells, suppressed berberine-induced cell death and expression of apoptosis-related proteins. In xenograft in vivo studies, berberine reduced tumor weights and volumes accompanied with apoptotic cell death and increased expression of apoptotic cell death proteins, however, the extent of inhibitory effect was more significant in LNCaP cell-bearing mice. Therefore, these results indicated that berberine inhibited p53-dependent prostate cancer cell death. |
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Authors:
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Myoung Suk Choi; Ju Hoon Oh; Sun Mi Kim; Hai Young Jung; Hwan Soo Yoo; Yong Moon Lee; Dong Cheul Moon; Sang Bae Han; Jin Tae Hong |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 34 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-10 Completed Date: 2009-08-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 1221-30 Citation Subset: IM |
Affiliation:
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College of Pharmacy and MRC, Chungbuk National University, Heungduk-gu, Cheongju, Chungbuk 361-763, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Berberine / pharmacology* Cell Cycle / drug effects, genetics Cell Proliferation* Cells, Cultured Down-Regulation / drug effects Humans Male Mice Mice, Inbred BALB C Mice, Nude Models, Biological Neoplasm Proteins / metabolism Prostatic Neoplasms / metabolism, pathology* Tumor Suppressor Protein p53 / metabolism, physiology* Xenograft Model Antitumor Assays |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/Tumor Suppressor Protein p53; 2086-83-1/Berberine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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