Document Detail


Berberine inhibits p53-dependent cell growth through induction of apoptosis of prostate cancer cells.
MedLine Citation:
PMID:  19360335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Berberine has anti-tumor properties in some cancer cells including prostate cancer, but the exact mechanisms and in vivo effects are unclear. We investigated anti-cancer activity of berberine in vitro and in vivo, and possible mechanisms in prostate cancer cells. Berberine treatment inhibited cell cancer growth in a concentration (0-50 microM) and time- (0-48 h) dependent manner without any growth inhibition in normal human prostate epithelial PWR-1E cells. However, the p53 expressing LNCaP cells were more susceptible against berberine than the p53 lacking PC-3 cells. The cell arrest in G0/G1 phase, apoptotic cell death and the expression of apoptotic cell death proteins Bax and caspase-3 was much higher in berberine-treated LNCaP cells than those in PC-3 cells. Exploration of p53 siRNA or pifithrin-alpha, a p53 inhibitor to the LNCaP cells, suppressed berberine-induced cell death and expression of apoptosis-related proteins. In xenograft in vivo studies, berberine reduced tumor weights and volumes accompanied with apoptotic cell death and increased expression of apoptotic cell death proteins, however, the extent of inhibitory effect was more significant in LNCaP cell-bearing mice. Therefore, these results indicated that berberine inhibited p53-dependent prostate cancer cell death.
Authors:
Myoung Suk Choi; Ju Hoon Oh; Sun Mi Kim; Hai Young Jung; Hwan Soo Yoo; Yong Moon Lee; Dong Cheul Moon; Sang Bae Han; Jin Tae Hong
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  34     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-10     Completed Date:  2009-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1221-30     Citation Subset:  IM    
Affiliation:
College of Pharmacy and MRC, Chungbuk National University, Heungduk-gu, Cheongju, Chungbuk 361-763, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Berberine / pharmacology*
Cell Cycle / drug effects,  genetics
Cell Proliferation*
Cells, Cultured
Down-Regulation / drug effects
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Models, Biological
Neoplasm Proteins / metabolism
Prostatic Neoplasms / metabolism,  pathology*
Tumor Suppressor Protein p53 / metabolism,  physiology*
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Tumor Suppressor Protein p53; 2086-83-1/Berberine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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