Document Detail


Berberine inhibits HEp-2 cell invasion induced by Chlamydophila pneumoniae infection.
MedLine Citation:
PMID:  22068503     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.
Authors:
Li Jun Zhang; Li Jun Zhang; Wei Quan; Bei Bei Wang; Bing Ling Shen; Teng Teng Zhang; Yi Kang
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Publication Detail:
Type:  Journal Article     Date:  2011-11-09
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  49     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  834-40     Citation Subset:  IM    
Affiliation:
Department of Pathophysiology, Basic Medical School of Tianjin Medical University, Tianjin, 300070, P. R. China.
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