| Berberine induces apoptosis through a mitochondrial/caspase pathway in human promonocytic U937 cells. | |
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MedLine Citation:
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PMID: 17011159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Berberine, an isoquinoline plant alkaloid, is known to generate a wide variety of biochemical and pharmacological effects. To elucidate the molecular mechanism of berberine-induced antiproliferative activities, the human promonocytic U937 cells were used. Berberine exhibited dose-dependent antiproliferative effects. Morphological evidence of apoptosis, including apoptotic DNA fragmentation, were observed in cells treated with 75 microg ml(-1) of berberine for 24h. Flow cytometry analysis revealed that berberine had no effect on cell cycle profile of U937 cells, however, sub-G(0) fraction (apoptotic cell population) was detected. The percentage of sub-G(0) fraction of cells treated with 75 microg ml(-1) of berberine was 25.3+/-1.6%. Berberine induces significant changes in mitochondrial membrane potential of U937 cells. The highest tested concentration of berberine decreased the mitochondrial membrane potential to 15.8+/-2.4% of control. Additionally, berberine-treated cells had an elevated level of ROS production. Activation of caspase-9 and caspase-3 was also detected, with no caspase-8 activation observed. Taken together, the results clearly demonstrate that berberine induces apoptosis of U937 cells through the mitochondrial/caspase-dependent pathway. |
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Authors:
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Sona Jantova; Lubos Cipak; Silvia Letasiova |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-25 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 21 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-04-09 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 25-31 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 81237 Bratislava, Slovakia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Berberine / pharmacology* Caspase 3 / metabolism, physiology Caspase 8 / metabolism, physiology Caspase 9 / metabolism, physiology Caspases / metabolism* Cell Cycle / drug effects Cell Proliferation / drug effects Cell Survival / drug effects DNA Fragmentation / drug effects Humans Membrane Potentials / drug effects Mitochondria / enzymology, metabolism* Monocytes / drug effects, enzymology, metabolism Reactive Oxygen Species / metabolism Signal Transduction / drug effects* U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 2086-83-1/Berberine; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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