Document Detail

Berberine-induced morphologic differentiation and down-regulation of c-Ki-ras2 protooncogene expression in human teratocarcinoma cells.
MedLine Citation:
PMID:  2265407     Owner:  NLM     Status:  MEDLINE    
A pluripotent human teratocarcinoma cell clone, NT2/D1, which was derived from the Tera-2 cell line, was induced to differentiate into cells with neuronal cell morphology by treatment with berberine. As early as 1 day after a 24-h treatment of cells with berberine at a non-toxic dose of 0.1 mg/ml in culture medium, the cells started to show morphologic changes, developing into terminally differentiated neuronal cells with long, inter-connecting network-like cellular structures. This process is much faster as compared with that induced by treatment with retinoic acid (RA), which took at least several days to develop. Unlike RA, berberine could not induce murine teratocarcinoma cell line, F9, to differentiate into endodermal cells. It was also found that, although the NT2/D1 cell clone exhibited amplification and enhanced mRNA expression of c-Ki-ras2 gene as did the parent cell line, a marked down-regulation of c-Ki-ras2 mRNA expression was observed. However, there was no change in actin mRNA expression even after differentiation had occurred. Thus, morphologic differentiation of teratocarcinoma cells into neuronal cells is found to be associated with down-regulation of a protooncogene which plays some definite role in oncogenesis. The mechanism by which berberine induces differentiation in these cells needs further investigation.
K S Chang; C Gao; L C Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  55     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-02-12     Completed Date:  1991-02-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  103-8     Citation Subset:  IM    
Laboratory of Cellular Oncology, National Cancer Institute, NIH, Bethesda, MD 20892.
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MeSH Terms
Berberine / pharmacology*
Cell Differentiation / drug effects*
Down-Regulation / drug effects*,  genetics
Gene Amplification
Gene Expression / drug effects
Oncogenes / genetics
Proto-Oncogenes / genetics*
Teratoma / genetics*,  pathology
Tumor Cells, Cultured
Reg. No./Substance:

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