| Beraprost sodium attenuates cigarette smoke extract-induced apoptosis in vascular endothelial cells. | |
| | |
MedLine Citation:
|
PMID: 23053949 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Apoptosis is now widely recognized as an important part of chronic obstructive pulmonary disease (COPD) pathogenesis. Our previous study demonstrated that a prostacyclin (PGI(2)) analogue (beraprost sodium, BPS) prevented cigarette smoke extract (CSE) induced apoptosis of the pulmonary endothelium in rats. So we determined to clarify the apoptosis of vascular endothelial cells in COPD patient and the role of prostacyclin in the protection against apoptosis in vascular endothelial cells induced by CSE. Surgical specimens were obtained from 12 patients with COPD and 10 controls, and the level of apoptosis, prostacyclin synthase (PGI(2)S) expression and 6-keto-PGF1α (a stable metabolite of PGI(2)) were detected. The apoptotic index (AI), caspase-3 activity, expression of caspase-3 and 6-keto-PGF1α were examined in human umbilical vein endothelial cells (HUVECs) under exposure to varied concentrations of CSE for 24 h as well as under exposure to 2.5 % CSE for varied durations. Then, HUVECs under 2.5 % CSE were exposed to varied concentrations of BPS for 24 h and observed the alteration and the level of cAMP. Increased AI, decreased expression of PGI(2)S and 6-keto-PGF1α, were found in the lungs of patients with COPD compared with controls. Moreover, CSE induced apoptosis in means of both dose-dependent and time-dependent manners, and reduced the level of 6-keto-PGF1α in HUVECs. And with the treatment of BPS, an enhanced level of cAMP and decreased apoptosis were detected. The deficiency of PGI(2) critically contributes to the COPD-associated endothelial dysfunction and apoptosis. And BPS protects against the apoptosis in the vascular endothelial cells induced by CSE. |
| | |
Authors:
|
Yan Chen; Hong Luo; Naixin Kang; Chaxiang Guan; Yingjiao Long; Jun Cao; Qin Shen; Junli Li; Min Yang; Hong Peng; Ping Chen |
Related Documents
:
|
14592969 - Ve-statin, an endothelial repressor of smooth muscle cell migration. 23180369 - Antiapoptotic and proapoptotic signaling of cyclophilin a in endothelial cells. 17150329 - Selenium and sulforaphane modify the expression of selenoenzymes in the human endotheli... 18310229 - Reversal of elastase-induced pulmonary emphysema and promotion of alveolar epithelial c... 16439709 - Increased serum cadmium and strontium levels in young smokers: effects on arterial endo... 23232839 - Endothelial dysfunction in diabetes and hypertension: cross talk in ras, bmp4 and ros-d... 3356579 - Intramural hematoma in the jejunum of a mare. 16953819 - Induction of the myofibroblastic phenotype in human gingival fibroblasts by transformin... 18947309 - Endothelial progenitor cells and their potential therapeutic applications. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-11 |
Journal Detail:
|
Title: Molecular biology reports Volume: - ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Division of Respiratory Disease, Department of Internal Medicine, The Second Xiangya Hospital, Central-South University, No. 139 Middle Renmin Road, Changsha, 410011, Hunan, China, chenyan727@yahoo.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Molecular characterization of differentially expressed TXNIP gene and its association with porcine c...
Next Document: A 5'UTR SNP of GHRHR locus is associated with body weight and average daily gain in Chinese cattle.