Document Detail


Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.
MedLine Citation:
PMID:  8670109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin-1 beta converting enzyme (ICE)-like proteases, which are synthesized as inactive precursors, play a key role in the induction of apoptosis. We now demonstrate that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits apoptosis by preventing the processing of CPP32 to its active form. These results suggest that novel inhibitors of apoptosis can be developed which prevent processing of proforms of ICE-like proteases.
Authors:
E A Slee; H Zhu; S C Chow; M MacFarlane; D W Nicholson; G M Cohen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  315 ( Pt 1)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-08-05     Completed Date:  1996-08-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  21-4     Citation Subset:  IM    
Affiliation:
MRC Toxicology Unit, University of Leicester, Lancaster Road, Leicester, U.K.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Chloromethyl Ketones / pharmacology*
Amino Acid Sequence
Apoptosis / drug effects*
Caspase 3
Caspases*
Cysteine Endopeptidases / drug effects*,  metabolism*
Cysteine Proteinase Inhibitors / pharmacology
Enzyme Activation / drug effects
Humans
Leukemia, Monocytic, Acute / enzymology,  pathology
Leukemia, T-Cell / enzymology,  pathology
Molecular Sequence Data
Oligopeptides / pharmacology
Poly(ADP-ribose) Polymerases / antagonists & inhibitors,  metabolism
Protease Inhibitors / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Amino Acid Chloromethyl Ketones; 0/Cysteine Proteinase Inhibitors; 0/Oligopeptides; 0/Protease Inhibitors; 0/acetyl-aspartyl-glutamyl-valyl-aspartal; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC 3.4.22.-/Cysteine Endopeptidases
Comments/Corrections

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