| Benzyl isothiocyanate (BITC) inhibits migration and invasion of human gastric cancer AGS cells via suppressing ERK signal pathways. | |
| | |
MedLine Citation:
|
PMID: 20498032 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Metastasis suppressors and associated other regulators of cell motility play a critical initial role in tumor invasion and metastases. Benzyl isothiocyanate (BITC) is a hydrolysis compound of glucotropaeolin in dietary cruciferous vegetables. BITC has been found to exhibit prevention of cancers in laboratory animals and might also be chemoprotective in humans. Here, the purpose of this study was to investigate the effects of BITC on cell proliferation, migration, invasion and mitogen-activated protein kinase (MAPK) pathways of AGS human gastric cancer cells. Wound healing and Boyden chamber (migration and invasion) assays demonstrated that BITC exhibited an inhibitory effect on the abilities of migration and invasion in AGS cancer cells. BITC suppressed cell migration and invasion of AGS cells in a dose-dependent manner. Results from Western blotting indicated that BITC exerted an inhibitory effect on the ERK1/2, Ras, GRB2, Rho A, iNOS, COX-2 for causing the inhibitions of MMP-2, -7 and -9 then followed by the inhibitions of invasion and migration of AGS cells in vitro. BITC also promoted MKK7, MEKK3, c-jun, JNK1/2, VEGF, Sos1, phosphoinositide 3-kinase (PI3K), PKC, nuclear factor-kappaB (NF-κB) p65 in AGS cells. Results from real-time polymerized chain reaction (PCR) showed that BITC inhibited the gene expressions of MMP-2,-7 -9, FAK, ROCK1 and RhoA after BITC treatment for 24 and 48 hours in AGS cells. Taken together, the finding may provide new mechanisms and functions of BITC, which inhibit migration and invasion of human gastric cancer AGS cells. |
| | |
Authors:
|
Chin-Chin Ho; Kuang-Chi Lai; Shu-Chun Hsu; Chao-Lin Kuo; Chia-Yu Ma; Meng-Liang Lin; Jai-Sing Yang; Jing-Gung Chung |
Publication Detail:
|
Type: Journal Article Date: 2010-05-24 |
Journal Detail:
|
Title: Human & experimental toxicology Volume: 30 ISSN: 1477-0903 ISO Abbreviation: Hum Exp Toxicol Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-03-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9004560 Medline TA: Hum Exp Toxicol Country: England |
Other Details:
|
Languages: eng Pagination: 296-306 Citation Subset: IM |
Affiliation:
|
Department of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of growth factors in acute lung injury induced by paraquat in a rat model.
Next Document: Toxicological evaluation of aqueous extract of Aloe ferox Mill. in loperamide-induced constipated ra...