| Benzoyl peroxide increases UVA-induced plasma membrane damage and lipid oxidation in murine leukemia L1210 cells. | |
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MedLine Citation:
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PMID: 9424093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ultraviolet A radiation induces oxidative stress and cell damage. The purpose of this investigation was to examine whether ultraviolet A-induced cell injury was amplified by the presence of a non-ultraviolet A absorbing molecule capable of generating free radicals. Benzoyl peroxide was used as a lipid soluble potential radical-generating agent. Plasma membrane permeability assessed by trypan blue uptake was used to measure cell damage in murine leukemia L1210 cells. Cells were irradiated with a pulsed Nd/YAG laser at 355 nm using 0-160 J per cm2. The ratio of the fluence-response slope in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.3 +/- 2.6. Benzoyl peroxide alone or benzoyl peroxide added after irradiation did not cause increased trypan blue uptake. The ratio of the fluence-response slopes in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.7 +/- 1.4 when cells were irradiated (0-43 J per cm2) with a xenon lamp, filtered to remove wavelengths <320 nm. The increased trypan blue uptake in 355 nm-irradiated cells in the presence of benzoyl peroxide was inhibited in a concentration-dependent manner by butylated hydroxytoluene, vitamin E, and trolox, a water-soluble vitamin E derivative. Lipid oxidation, assessed as thiobarbituric acid reactive substances, was significantly increased in samples irradiated with ultraviolet A in the presence of benzoyl peroxide at fluences >34 J per cm2. The increased trypan blue uptake and thiobarbituric acid reactive substances were inhibited by butylated hydroxytoluene. These results suggest that agents not absorbing ultraviolet A radiation may enhance ultraviolet A-initiated oxidative stress in cells. |
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Authors:
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S H Ibbotson; C R Lambert; M N Moran; M C Lynch; I E Kochevar |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 110 ISSN: 0022-202X ISO Abbreviation: J. Invest. Dermatol. Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-01-22 Completed Date: 1998-01-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 79-83 Citation Subset: IM |
Affiliation:
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Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption
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drug effects,
radiation effects Animals Antioxidants / pharmacology Benzoyl Peroxide / pharmacokinetics, pharmacology* Butylated Hydroxytoluene / pharmacology Cell Membrane / radiation effects*, ultrastructure Cell Membrane Permeability / drug effects Lasers Leukemia L1210 / pathology* Membrane Lipids / metabolism* Mice Oxidation-Reduction Oxidative Stress / drug effects*, radiation effects* Thiobarbituric Acid Reactive Substances / metabolism Ultraviolet Rays* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM30755/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Membrane Lipids; 0/Thiobarbituric Acid Reactive Substances; 128-37-0/Butylated Hydroxytoluene; 94-36-0/Benzoyl Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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