Document Detail


Benzobijuglone, a novel cytotoxic compound from Juglans mandshurica, induced apoptosis in HeLa cervical cancer cells.
MedLine Citation:
PMID:  17959366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new quinone compound, p-hydroxymethoxybenzobijuglone (HMBBJ), isolated from Juglans mandshurica by bioassay-guided fractionation, showed cytotoxic activity against HeLa cell line. Its chemical structure was determined by NMR and HREIMS spectra. In this paper, its ability to induce apoptosis in HeLa cells was studied for the first time. After treated with HMBBJ, the growth of HeLa cells was inhibited and cells displayed typical morphological apoptotic characteristics. Data from flow cytometry analysis showed that the HeLa cell cycle was arrested in the G2/M phase by HMBBJ, and the apoptotic rate of HeLa cells increased in a dose-dependent manner. Meanwhile, HMBBJ increased the expression of caspase-8, -3 and Bax, decreased the expression of Bcl-2, and lowered the DeltaPsi(m). These findings reveal that HMBBJ could efficiently induce HeLa cells apoptosis through mitochondria dependent pathway and activation of the caspase cascade, and it may be a potential chemotherapeutic candidate for the treatment of cancer.
Authors:
Zhi-Bo Li; Jing-Yun Wang; Bo Jiang; Xiu-Li Zhang; Li-Jia An; Yong-Ming Bao
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Publication Detail:
Type:  Journal Article     Date:  2007-10-23
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  14     ISSN:  0944-7113     ISO Abbreviation:  Phytomedicine     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-05     Completed Date:  2008-02-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  846-52     Citation Subset:  IM    
Affiliation:
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, PR China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Benzoquinones / pharmacology*
Caspase 3 / metabolism
Caspase 8 / metabolism
Female
Flow Cytometry
Hela Cells
Humans
Membrane Potentials / drug effects
Mitochondria / drug effects,  physiology
Proto-Oncogene Proteins c-bcl-2 / metabolism
Uterine Cervical Neoplasms / pathology
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/p-hydroxymethoxybenzobijuglone; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8

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