Document Detail

Benfotiamine attenuates nicotine and uric acid-induced vascular endothelial dysfunction in the rat.
MedLine Citation:
PMID:  18951979     Owner:  NLM     Status:  MEDLINE    
The study has been designed to investigate the effect of benfotiamine, a thiamine derivative, in nicotine and uric acid-induced vascular endothelial dysfunction (VED) in rats. Nicotine (2 mg kg(-1)day(-1), i.p., 4 weeks) and uric acid (150 mg kg(-1)day(-1), i.p., 3 weeks) were administered to produce VED in rats. The development of VED was assessed by employing isolated aortic ring preparation and estimating serum and aortic concentration of nitrite/nitrate. Further, the integrity of vascular endothelium was assessed using the scanning electron microscopy (SEM) of thoracic aorta. Moreover, the oxidative stress was assessed by estimating serum thiobarbituric acid reactive substances (TBARS) and aortic superoxide anion generation. The administration of nicotine and uric acid produced VED by impairing the integrity of vascular endothelium and subsequently decreasing serum and aortic concentration of nitrite/nitrate and attenuating acetylcholine-induced endothelium dependent relaxation. Further, nicotine and uric acid produced oxidative stress, which was assessed in terms of increase in serum TBARS and aortic superoxide generation. However, treatment with benfotiamine (70 mg kg(-1)day(-1), p.o.) or atorvastatin (30 mg kg(-1)day(-1) p.o., a standard agent) markedly prevented nicotine and uric acid-induced VED and oxidative stress by improving the integrity of vascular endothelium, increasing the concentration of serum and aortic nitrite/nitrate, enhancing the acetylcholine-induced endothelium dependent relaxation and decreasing serum TBARS and aortic superoxide anion generation. Thus, it may be concluded that benfotiamine reduces the oxidative stress and consequently improves the integrity of vascular endothelium and enhances the generation of nitric oxide to prevent nicotine and uric acid-induced experimental VED.
Pitchai Balakumar; Ramica Sharma; Manjeet Singh
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2008-10-02
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  58     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  356-63     Citation Subset:  IM    
Cardiovascular Pharmacology Division, ISF College of Pharmacy, Moga 142001, Punjab, India.
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MeSH Terms
Aorta, Thoracic / drug effects
Endothelium, Vascular / drug effects*
Heptanoic Acids / pharmacology
Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology
Microscopy, Electron, Scanning
Nicotine / antagonists & inhibitors*,  toxicity*
Nicotinic Agonists / toxicity*
Nitrates / blood,  metabolism
Nitrites / blood,  metabolism
Nitroprusside / pharmacology
Oxidative Stress / drug effects
Pyrroles / pharmacology
Rats, Wistar
Superoxides / metabolism
Thiamine / analogs & derivatives*,  pharmacology
Thiobarbituric Acid Reactive Substances / metabolism
Uric Acid / antagonists & inhibitors*,  toxicity*,  urine
Vascular Diseases / chemically induced*,  prevention & control*
Vasodilation / drug effects,  physiology
Reg. No./Substance:
0/Heptanoic Acids; 0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Nicotinic Agonists; 0/Nitrates; 0/Nitrites; 0/Pyrroles; 0/Thiobarbituric Acid Reactive Substances; 11062-77-4/Superoxides; 110862-48-1/atorvastatin; 15078-28-1/Nitroprusside; 22457-89-2/benphothiamine; 54-11-5/Nicotine; 59-43-8/Thiamine; 69-93-2/Uric Acid

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