| Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. | |
| | |
MedLine Citation:
|
PMID: 19422396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND AND PURPOSE: Elevated plasma total homocysteine (tHcy) level has been established as an independent risk factor for cardiovascular diseases. Danshensu, an active ingredient of Salvia miltiorrhiza, shows wide cardiovascular benefit. However, in terms of its own methylation, danshensu could elevate tHcy level, which would act against its cardiovascular benefit, thus posing a 'therapeutic paradox'. As this paradox has not been fully assessed, we have evaluated the effects of danshensu on tHcy levels to uncover the underlying mechanisms. EXPERIMENT APPROACH: We evaluated the influence of danshensu on homocysteine (Hcy) metabolism in rats with normal tHcy levels and in rat models of elevated tHcy (single intravenous methionine loading model and a hyperhomocysteinemic model after 3 weeks methionine dosing, with and without 3 weeks of danshensu treatment). We also quantified some metabolic intermediates (S-adenosyl methionine, S-adenosyl-l-homocysteine, cysteine and glutathione) relevant to Hcy metabolism in rat liver and kidney. KEY RESULTS: Acute treatment with a single dose of danshensu in rats with normal tHcy did not change plasma tHcy. In contrast, danshensu significantly lowered tHcy in rats with elevated tHcy. The relatively higher cysteine and glutathione levels after treatment with danshensu indicated that its tHcy-lowering effect was via increased activity of the trans-sulphuration pathway. CONCLUSIONS AND IMPLICATIONS: Our results suggested that danshensu may act both acutely to increase trans-sulphuration and after chronic exposure to up-regulate the activity of the trans-sulphuration enzymes. The tHcy-lowering effect of danshensu is another cardiovascular benefit provided by S. miltiorrhiza and suggests a potential tHcy-lowering therapy. |
| | |
Authors:
|
Yg Cao; J G Chai; Y C Chen; J Zhao; J Zhou; J P Shao; C Ma; X D Liu; X Q Liu |
Related Documents
:
|
6743236 - Selective induction of glutathione s-transferase d in rat testis by phenobarbital. 22508486 - Effects of glycyrrhizin on biliary transport and hepatic levels of glutathione in rats. 18098036 - Diuron lacks promoting potential in a rat liver bioassay. 11460726 - Altered glutathione s-transferase catalytic activities in female brown bullheads from a... 6805486 - The effects of early-life undernutrition and subsequent environment on morphological pa... 14634456 - Experimental autoimmune prostatitis: dihydrotestosterone influence over the immune resp... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-30 |
Journal Detail:
|
Title: British journal of pharmacology Volume: 157 ISSN: 1476-5381 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-06-17 Completed Date: 2009-10-14 Revised Date: 2010-09-27 |
Medline Journal Info:
|
Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
|
Languages: eng Pagination: 482-90 Citation Subset: IM |
Affiliation:
|
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cardiovascular Agents / pharmacology*, therapeutic use Cysteine / blood Dose-Response Relationship, Drug Glutathione / blood Homocysteine / blood* Hyperhomocysteinemia / blood, drug therapy Lactates / pharmacology*, therapeutic use Male Methionine Rats Rats, Sprague-Dawley Salvia miltiorrhiza / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Cardiovascular Agents; 0/Lactates; 23028-17-3/3,4-dihydroxyphenyllactic acid; 454-28-4/Homocysteine; 52-90-4/Cysteine; 63-68-3/Methionine; 70-18-8/Glutathione |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of G12 proteins in oncogenesis and metastasis.
Next Document: The hepoxilins and some analogues: a review of their biology.